Please use this identifier to cite or link to this item: https://doi.org/10.1007/BF00023395
Title: Three genes encode 3-hydroxy-3-methylglutaryl-coenzyme A reductase in Hevea brasiliensis: hmg1 and hmg3 are differentially expressed
Authors: Chye, M.-L. 
Tan, C.-T. 
Chua, N.-H.
Keywords: cis-1,4-polyisoprene
ethylene induction
isoprenoid biosynthesis
latex
laticifer
natural rubber
Issue Date: Jun-1992
Citation: Chye, M.-L., Tan, C.-T., Chua, N.-H. (1992-06). Three genes encode 3-hydroxy-3-methylglutaryl-coenzyme A reductase in Hevea brasiliensis: hmg1 and hmg3 are differentially expressed. Plant Molecular Biology 19 (3) : 473-484. ScholarBank@NUS Repository. https://doi.org/10.1007/BF00023395
Abstract: The enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) catalyses an important step in isoprenoid biosynthesis in plants. In Hevea brasiliensis, HMGR is encoded by a small gene family comprised of three members, hmg1, hmg2 and hmg3. We have previously described hmg1 and hmg2 (Plant Mol Biol 16: 567-577, 1991). Here we report the isolation and characterization of hmg3 genomic and cDNA clones. In comparison to hmg1 which is more highly expressed in laticifers than in leaves, the level of hmg3 mRNA level is equally abundant in laticifers and leaves. In situ hybridization experiments showed that the expression of hmg3 is not cell-type specific while hmg1 is expressed predominantly in the laticifers. Primer-extension experiments using laticifer RNA showed that hmg1 is induced by ethylene while hmg3 expression remains constitutive. The hmg3 promoter, like the promoters of most house-keeping genes, lacks a TATA box. Our results suggest that hmg1 is likely to encode the enzyme involved in rubber biosynthesis while hmg3 is possibly involved in isoprenoid biosynthesis of a housekeeping nature. © 1992 Kluwer Academic Publishers.
Source Title: Plant Molecular Biology
URI: http://scholarbank.nus.edu.sg/handle/10635/112139
ISSN: 01674412
DOI: 10.1007/BF00023395
Appears in Collections:Staff Publications

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