Please use this identifier to cite or link to this item:
https://doi.org/10.1006/bbrc.1998.8323
DC Field | Value | |
---|---|---|
dc.title | Mutations which abolish phosphorylation of the TRAF-binding domain of TNF receptor 2 enhance receptor-mediated NF-κB activation | |
dc.contributor.author | Ng, P.W.P. | |
dc.contributor.author | Jänicke, R.U. | |
dc.contributor.author | Porter, A.G. | |
dc.date.accessioned | 2014-11-28T02:51:52Z | |
dc.date.available | 2014-11-28T02:51:52Z | |
dc.date.issued | 1998-03-27 | |
dc.identifier.citation | Ng, P.W.P., Jänicke, R.U., Porter, A.G. (1998-03-27). Mutations which abolish phosphorylation of the TRAF-binding domain of TNF receptor 2 enhance receptor-mediated NF-κB activation. Biochemical and Biophysical Research Communications 244 (3) : 756-762. ScholarBank@NUS Repository. https://doi.org/10.1006/bbrc.1998.8323 | |
dc.identifier.issn | 0006291X | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/111985 | |
dc.description.abstract | We demonstrate that a 41 amino acid region (amino acids 379 to 419) in the cytoplasmic domain of tumor necrosis factor receptor 2 (TNFR2) is phosphorylated by unidentified kinase(s) both in vitro and in vivo. This domain (denoted x1c) corresponds almost exactly to the previously identified TRAF-binding domain and is by itself sufficient as a substrate for phosphorylation. In addition, the x1c domain is also crucial for TNFR2-mediated NF-κB activation. The cytoplasmic domain of TNFR2 lacks tyrosines, and conversion of all 12 potential serine and threonine phosphorylation targets in x1c to alanines either had no effect on NF-κB activation or resulted in enhanced NF-κB activity, depending on the structural context of x1c. The results show that while the TRAF-binding domain of TNFR2 is a major target of kinases, its phosphorylation is not required for NF-κB activation. Our data moreover suggest that phosphorylation of x1c negatively regulates the activation of NF-κB. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1006/bbrc.1998.8323 | |
dc.source | Scopus | |
dc.subject | NF-κB activation | |
dc.subject | Phosphorylation | |
dc.subject | TNF receptor | |
dc.subject | TRAF-binding domain | |
dc.type | Article | |
dc.contributor.department | INSTITUTE OF MOLECULAR & CELL BIOLOGY | |
dc.description.doi | 10.1006/bbrc.1998.8323 | |
dc.description.sourcetitle | Biochemical and Biophysical Research Communications | |
dc.description.volume | 244 | |
dc.description.issue | 3 | |
dc.description.page | 756-762 | |
dc.description.coden | BBRCA | |
dc.identifier.isiut | 000072919100026 | |
Appears in Collections: | Staff Publications |
Show simple item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.