Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/111801
Title: Binding of encephalomyocarditis virus RNA polymerase to the 3′-noncoding region of the viral RNA is specific and requires the 3′-poly(A) tail
Authors: Cui, T. 
Sankar, S. 
Porter, A.G. 
Issue Date: 15-Dec-1993
Source: Cui, T.,Sankar, S.,Porter, A.G. (1993-12-15). Binding of encephalomyocarditis virus RNA polymerase to the 3′-noncoding region of the viral RNA is specific and requires the 3′-poly(A) tail. Journal of Biological Chemistry 268 (35) : 26093-26098. ScholarBank@NUS Repository.
Abstract: An in vitro RNA bandshift assay has been developed to demonstrate the binding of purified recombinant encephalomyocarditis (EMC) virus RNA-dependent RNA polymerase (3Dpol) to the 3′-noncoding region (NCR) and 30 nucleotides of the adjacent 3′-terminal poly(A) tail (3′-NCR(A)) of EMC virus RNA. The binding of 3Dpol to the 3′-NCR(A) fragment was specific since four other unrelated proteins including an RNA polymerase did not bind, and unlabeled 3′-NCR(A), but not α-globin mRNA, tRNA, or pure poly(A), competed with radiolabeled 3′-NCR(A) for binding. Surprisingly, 3Dpol failed to bind to the 3′-NCR of EMC virus RNA lacking the poly(A) tail. The results together show that EMC virus RNA template specificity depends only on 3Dpol, the 3′-NCR, and the poly(A) tail. This suggests that 3′-poly(A) is essential for viral RNA template selection by the EMC virus RNA polymerase.
Source Title: Journal of Biological Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/111801
ISSN: 00219258
Appears in Collections:Staff Publications

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