Please use this identifier to cite or link to this item:
|Title:||Binding of encephalomyocarditis virus RNA polymerase to the 3′-noncoding region of the viral RNA is specific and requires the 3′-poly(A) tail|
|Authors:||Cui, T. |
|Source:||Cui, T.,Sankar, S.,Porter, A.G. (1993-12-15). Binding of encephalomyocarditis virus RNA polymerase to the 3′-noncoding region of the viral RNA is specific and requires the 3′-poly(A) tail. Journal of Biological Chemistry 268 (35) : 26093-26098. ScholarBank@NUS Repository.|
|Abstract:||An in vitro RNA bandshift assay has been developed to demonstrate the binding of purified recombinant encephalomyocarditis (EMC) virus RNA-dependent RNA polymerase (3Dpol) to the 3′-noncoding region (NCR) and 30 nucleotides of the adjacent 3′-terminal poly(A) tail (3′-NCR(A)) of EMC virus RNA. The binding of 3Dpol to the 3′-NCR(A) fragment was specific since four other unrelated proteins including an RNA polymerase did not bind, and unlabeled 3′-NCR(A), but not α-globin mRNA, tRNA, or pure poly(A), competed with radiolabeled 3′-NCR(A) for binding. Surprisingly, 3Dpol failed to bind to the 3′-NCR of EMC virus RNA lacking the poly(A) tail. The results together show that EMC virus RNA template specificity depends only on 3Dpol, the 3′-NCR, and the poly(A) tail. This suggests that 3′-poly(A) is essential for viral RNA template selection by the EMC virus RNA polymerase.|
|Source Title:||Journal of Biological Chemistry|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jan 19, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.