A novel method employing polymerase chain reaction to disrupt genes lacking convenient restriction enzyme sites in yeast

Please use this identifier to cite or link to this item: https://doi.org/10.1007/BF02821336

Title:	A novel method employing polymerase chain reaction to disrupt genes lacking convenient restriction enzyme sites in yeast
Authors:	Wu, M. Shang, H.-S. Yeong, C.-Y. Tan, P.H.-N. Lu, Z.-X. Wong, S.-M.
Keywords:	gene disruption Polymerase chain reaction yeast
Issue Date:	Feb-1995
Citation:	Wu, M., Shang, H.-S., Yeong, C.-Y., Tan, P.H.-N., Lu, Z.-X., Wong, S.-M. (1995-02). A novel method employing polymerase chain reaction to disrupt genes lacking convenient restriction enzyme sites in yeast. Molecular Biotechnology 3 (1) : 72-74. ScholarBank@NUS Repository. https://doi.org/10.1007/BF02821336
Abstract:	A novel method employing polymerase chain reaction was developed for the disruption of yeast genes lacking convenient restriction enzyme sites. The method was found to be easy and effective. Using this method, a yeast YKE2 gene (a yeast homolog of murine k-region expressed genes) was successfully disrupted by replacement of HIS3 marker gene. © 1995 Humana Press Inc.
Source Title:	Molecular Biotechnology
URI:	http://scholarbank.nus.edu.sg/handle/10635/111764
ISSN:	10736085
DOI:	10.1007/BF02821336
Appears in Collections:	Staff Publications

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