Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/111747
DC Field | Value | |
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dc.title | 2,6-Dichloro-4-nitrophenol (DCNP), an alternate-substrate inhibitor of phenolsulfotransferase | |
dc.contributor.author | Seah, V.M.Y. | |
dc.contributor.author | Kim Ping Wong | |
dc.date.accessioned | 2014-11-28T02:49:13Z | |
dc.date.available | 2014-11-28T02:49:13Z | |
dc.date.issued | 1994-05-18 | |
dc.identifier.citation | Seah, V.M.Y.,Kim Ping Wong (1994-05-18). 2,6-Dichloro-4-nitrophenol (DCNP), an alternate-substrate inhibitor of phenolsulfotransferase. Biochemical Pharmacology 47 (10) : 1743-1749. ScholarBank@NUS Repository. | |
dc.identifier.issn | 00062952 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/111747 | |
dc.description.abstract | 2,6-Dichloro-4-nitrophenol (DCNP)-35sulfate was identified and quantified by an HPLC-radiometric assay following its biosynthesis in vitro from 35S-labeled 3'-phosphoadenosine-5'-phosphosulfate (PAP35S) by phenolsulfotransferase (PST) of rat liver cytosol. Acid hydrolysis of DCNP-35sulfate produced almost stoichiometric release of inorganic 35sulfate and DCNP. In two-substrate experiments of sulfation of p-nitrophenol (p-NP) or dopamine (prototype substrates for P and M human PST forms), 10 μM DCNP inhibited the reactions by about 15 and 78%, respectively. This contrasts with its action on PST of human origin where the P-PST was more sensitive to DCNP inhibition. In all mixed bi-substrate experiments, a reciprocal relationship between the two sulfated products was observed. Kinetic data showed that p-NP inhibited the sulfation of DCNP competitively. Likewise the sulfation of p-NP and dopamine was competitively inhibited by DCNP. However, non-competitive inhibition was observed in the sulfation of p-NP by DCNP, measured at varying concentrations of PAP35S. The above kinetic data suggest that DCNP is an alternate-substrate inhibitor of rat liver PST. © 1994. | |
dc.source | Scopus | |
dc.subject | DCNP | |
dc.subject | dopamine | |
dc.subject | phenolsulfotransferase (PST) | |
dc.subject | rat liver | |
dc.type | Article | |
dc.contributor.department | INSTITUTE OF MOLECULAR & CELL BIOLOGY | |
dc.description.sourcetitle | Biochemical Pharmacology | |
dc.description.volume | 47 | |
dc.description.issue | 10 | |
dc.description.page | 1743-1749 | |
dc.description.coden | BCPCA | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Staff Publications |
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