Please use this identifier to cite or link to this item: https://doi.org/10.1006/abio.1993.1557
Title: Pyridylethylation of cysteine residues in proteins
Authors: Kao, M.C.C. 
Chung, M.C.M.
Issue Date: 1993
Source: Kao, M.C.C., Chung, M.C.M. (1993). Pyridylethylation of cysteine residues in proteins. Analytical Biochemistry 215 (1) : 82-85. ScholarBank@NUS Repository. https://doi.org/10.1006/abio.1993.1557
Abstract: A procedure is described for using an inert polyvinylidene difluoride type membrane (e.g., ProBlott) as a matrix for on-membrane gas-phase pyridylethylation and as a desalting (cleanup) support following the solution phase pyridylethylation of cysteine residues in proteins. As a desalting support for 200 pmol of cardiotoxin, the membrane-derived sample gave a better recovery of phenylthiohydantoin (PTH)-amino acids during N-terminal sequencing compared to the sample obtained bv rpHPLC. Using on-membrane gas-phase pyridylethylation, followed by replacement of glassfiber filter disc and membrane washing/drying in vacuo, it was possible to eliminate the broad background peak that coeluted with PTH-Ala in the HPLC chromatogram completely. Using this modified procedure, there was no evidence of N-terminal alkylation of the amino-termini of proteins.
Source Title: Analytical Biochemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/111048
ISSN: 00032697
DOI: 10.1006/abio.1993.1557
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