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|Title:||Pyridylethylation of cysteine residues in proteins|
|Authors:||Kao, M.C.C. |
|Citation:||Kao, M.C.C., Chung, M.C.M. (1993). Pyridylethylation of cysteine residues in proteins. Analytical Biochemistry 215 (1) : 82-85. ScholarBank@NUS Repository. https://doi.org/10.1006/abio.1993.1557|
|Abstract:||A procedure is described for using an inert polyvinylidene difluoride type membrane (e.g., ProBlott) as a matrix for on-membrane gas-phase pyridylethylation and as a desalting (cleanup) support following the solution phase pyridylethylation of cysteine residues in proteins. As a desalting support for 200 pmol of cardiotoxin, the membrane-derived sample gave a better recovery of phenylthiohydantoin (PTH)-amino acids during N-terminal sequencing compared to the sample obtained bv rpHPLC. Using on-membrane gas-phase pyridylethylation, followed by replacement of glassfiber filter disc and membrane washing/drying in vacuo, it was possible to eliminate the broad background peak that coeluted with PTH-Ala in the HPLC chromatogram completely. Using this modified procedure, there was no evidence of N-terminal alkylation of the amino-termini of proteins.|
|Source Title:||Analytical Biochemistry|
|Appears in Collections:||Staff Publications|
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