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|Title:||Continuous epitopes of human lymphotoxin and their topographies|
|Authors:||Yao, Z.-J. |
|Citation:||Yao, Z.-J.,Kao, M.C.C.,Loh, K.-C.,Chung, M.C.M. (1994). Continuous epitopes of human lymphotoxin and their topographies. Biochemistry and Molecular Biology International 32 (5) : 951-959. ScholarBank@NUS Repository.|
|Abstract:||Human lymphotoxin (hLT or TNF-β) is a lymphokine that is structurally and functionally related to tumor necrosis factor alpha (TNF-α). The continuous epitopes of hLT were located by examining the cross-reaction between rabbit anti-hLT antibody and peptides derived from proteolytic digestion and chemical synthesis. Three antigenic sites, corresponding to residues 40-48, 83-94 and 139-147, of the protein sequence, were located by this approach. Since residues 49-57 also exhibited trace antigenicity, but residues 45-52 displayed no reaction, the whole peptide fragment consisting of residues 40-57 might be necessary for antigenicity. A comparison of the antigenic determinants with the loop structures obtained from X-ray crystallographic studies of hLT showed that all of the epitopes are found on or adjacent to functionally important domains.|
|Source Title:||Biochemistry and Molecular Biology International|
|Appears in Collections:||Staff Publications|
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