Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/111034
Title: Antibody against branched epitope as an affinity ligand to separate the parent protein
Authors: Yao, Z.J. 
Kao, M.C.C. 
Loh, K.C. 
Chung, M.C.M.
Issue Date: 9-Sep-1994
Source: Yao, Z.J.,Kao, M.C.C.,Loh, K.C.,Chung, M.C.M. (1994-09-09). Antibody against branched epitope as an affinity ligand to separate the parent protein. Journal of Chromatography A 679 (1) : 190-194. ScholarBank@NUS Repository.
Abstract: A peptide that contained one of the continuous epitopes of recombinant human lymphotoxin (rhLT) (amino acid residues 139-154) has been located by epitope mapping. The branched form of this peptide was synthesized by the multiple antigen peptide procedure with an octameric branched resin and was subsequently used to elicit anti-epitope antibody in rabbits. The resulting anti-epitope was then used as an immunoaffinity ligand in affinity chromatography to purify the parent protein, rhLT, from the host cell lysate directly. It is suggested that this approach would be a general way to create novel biospecific ligands for affinity separations. © 1994.
Source Title: Journal of Chromatography A
URI: http://scholarbank.nus.edu.sg/handle/10635/111034
ISSN: 00219673
Appears in Collections:Staff Publications

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