Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0166-0934(02)00212-4
Title: Production and characterization of monoclonal antibodies to a grouper iridovirus
Authors: Shi, C.
Qin, Q.W. 
Gin, K.Y.H.
Lam, T.J. 
Keywords: Epinephelus spp.
Grouper
Iridovirus
Monoclonal antibodies
Issue Date: Feb-2003
Citation: Shi, C., Qin, Q.W., Gin, K.Y.H., Lam, T.J. (2003-02). Production and characterization of monoclonal antibodies to a grouper iridovirus. Journal of Virological Methods 107 (2) : 147-154. ScholarBank@NUS Repository. https://doi.org/10.1016/S0166-0934(02)00212-4
Abstract: A panel of six monoclonal antibodies (mAbs) against a grouper iridovirus (SGIV) were produced by immunization of Balb/c mice with purified virus preparations. Isotyping test revealed all the mAbs were IgG1. None of the mAbs possessed the ability to neutralize SGIV in cell cultures. Western blot showed that 4 mAbs reacted with 2 SGIV proteins at molecular mass of approximately 100 and 117 kDa in gradient-purified virus. Immunofluorescent studies showed that the two specific viral proteins VP100 and VP117 were localized within virus assembly sites in the cytoplasm of SGIV-infected grouper cells (GP). Fractionations of the iridovirus in a 20-60% sucrose gradient were detected successfully by all the six mAbs using immunodot blot. An antigen-capture enzyme-linked immunosorbent assay (ELISA) system, based on the use of mAb 7E11 for capture and a rabbit polyclonal antibody to SGIV for detection was developed. SGIV antigen was detected in gradient-purified virus and virus-infected grouper blood. These novel mAbs will facilitate the development of more specific and standardized diagnostic techniques for marine fish iridovirus. © 2002 Elsevier Science B.V. All rights reserved.
Source Title: Journal of Virological Methods
URI: http://scholarbank.nus.edu.sg/handle/10635/110893
ISSN: 01660934
DOI: 10.1016/S0166-0934(02)00212-4
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