Please use this identifier to cite or link to this item:
|Title:||Reduced plasma APOA1 level is associated with Gastric Tumor Growth in MKN45 mouse xenograft model|
|Authors:||Chong, P.-K. |
|Source:||Chong, P.-K., Lee, H., Zhou, J., Liu, S.-C., Loh, M.C.S., So, J.B.Y., Lim, K.H., Yeoh, K.-G., Lim, Y.-P. (2010-06-16). Reduced plasma APOA1 level is associated with Gastric Tumor Growth in MKN45 mouse xenograft model. Journal of Proteomics 73 (8) : 1632-1640. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jprot.2010.04.005|
|Abstract:||There is no suitable diagnostic and prognostic biomarker for gastric cancer. The biggest hurdles in biomarker discovery are (i) the low abundance of cancer cell-specific proteins that limits their detection and (ii) complex inter-patient variations that complicate the discovery process. To circumvent these issues, we conducted proteomics on the plasma of gastric cancer mouse xenograft and attempted to identify proteins released by cancer cells. MKN45 gastric cancer cells were subcutaneously implanted into immune-incompetent nude mice. Plasma samples collected from mice with different tumor sizes (low, mid and high tumor loads) were subjected to iTRAQ and mass spectrometric analyses. Detection of human APOA1 in mouse plasma was verified and its expression level was shown to be lower in mice with large tumors compared to those with small tumors. Studies on a panel of about 14 gastric cancer cell lines supported the notion that APOA1 in mouse plasma was of human gastric cancer cell origin. While the clinical utility of APOA1 remains to be ascertained with a larger scale study, the current work supported the feasibility of using mouse xenograft model for gastric cancer biomarker discovery. © 2010 Elsevier B.V. All rights reserved.|
|Source Title:||Journal of Proteomics|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Feb 28, 2018
WEB OF SCIENCETM
checked on Feb 19, 2018
checked on Apr 20, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.