Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.nano.2013.05.009
Title: Protective role of functionalized single walled carbon nanotubes enhance ex vivo expansion of hematopoietic stem and progenitor cells in human umbilical cord blood
Authors: Bari, S.
Chu, P.P.Y.
Lim, A.
Fan, X.
Gay, F.P.H.
Bunte, R.M. 
Lim, T.K.H.
Li, S.
Chiu, G.N.C. 
Hwang, W.Y.K.
Keywords: Ex Vivo Expansion of UCB
Functionalized Single Walled Carbon Nanotubes (f-SWNCT)
Hematopoietic Stem Cell Transplantation (HSCT)
Immunodeficient Mouse Model
Umbilical Cord Blood (UCB)
Issue Date: Nov-2013
Citation: Bari, S., Chu, P.P.Y., Lim, A., Fan, X., Gay, F.P.H., Bunte, R.M., Lim, T.K.H., Li, S., Chiu, G.N.C., Hwang, W.Y.K. (2013-11). Protective role of functionalized single walled carbon nanotubes enhance ex vivo expansion of hematopoietic stem and progenitor cells in human umbilical cord blood. Nanomedicine: Nanotechnology, Biology, and Medicine 9 (8) : 1304-1316. ScholarBank@NUS Repository. https://doi.org/10.1016/j.nano.2013.05.009
Abstract: In this study, carboxylic acid functionalized single walled carbon nanotubes (f-SWCNT-COOH) was shown to support the viability and ex vivo expansion of freeze-thawed, non-enriched hematopoietic stem and progenitor cells (HSPC) in human umbilical cord blood-mononucleated cells (UCB-MNC). Our in vitro experiments showed that f-SWCNT-COOH increased the viability of the CD45+ cells even without cytokine stimulation. It also reduced mitochondrial superoxides and caspase activity in CD45+ cells. f-SWCNT-COOH drastically reduced the proportions of CD45- cells in the non-enriched UCB-MNC. Phenotypic expression analysis and functional colony forming units (CFU) showed significant ex vivo expansion of HSPC, particularly of CD45+CD34+CD38- population and granulocyte-macrophage (GM) colonies, in f-SWCNT-COOH augmented cultures supplemented with basal cytokines. In vivo data suggested that f-SWCNT-COOH expanded UCB-MNC could repopulate immunodeficient mice models with minimal acute or sub-acute symptoms of graft-versus-host disease (GVHD) and f-SWCNT-COOH dependent toxicity. From the Clinical Editor: In this paper a novel method is presented by using single wall functionalized carbon nanotubes to enhance viability and ex vivo expansion of freeze-thawed, non-enriched hematopoietic stem and progenitor cells in human umbilical cord blood -mononucleated cells. Detailed data is presented about enhanced viability, including improved repopulation of immunodeficient mice models with minimal acute or sub-acute symptoms of graft-versus-host disease. © 2013 Elsevier Inc.
Source Title: Nanomedicine: Nanotechnology, Biology, and Medicine
URI: http://scholarbank.nus.edu.sg/handle/10635/110614
ISSN: 15499634
DOI: 10.1016/j.nano.2013.05.009
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