Please use this identifier to cite or link to this item: https://doi.org/10.1194/jlr.M044826
Title: Extensive characterization of human tear fl uid collected using different techniques unravels the presence of novel lipid amphiphiles
Authors: Lam, S.M.
Tong, L. 
Duan, X.
Petznick, A.
Wenk, M.R.
Shui, G.
Keywords: Cholesteryl sulfates
Dry eye syndrome
Lipidomics
Mass spectrometry
Meibum
O-Acyl- α - hydroxy fatty acids
Tear lipidome
Issue Date: Feb-2014
Citation: Lam, S.M., Tong, L., Duan, X., Petznick, A., Wenk, M.R., Shui, G. (2014-02). Extensive characterization of human tear fl uid collected using different techniques unravels the presence of novel lipid amphiphiles. Journal of Lipid Research 55 (2) : 289-298. ScholarBank@NUS Repository. https://doi.org/10.1194/jlr.M044826
Abstract: The tear film covers the anterior eye and the precise balance of its various constituting components is critical for maintaining ocular health. The composition of the tear film amphiphilic lipid sublayer, in particular, has largely remained a matter of contention due to the limiting concentrations of these lipid amphiphiles in tears that render their detection and accurate quantitation tedious. Using systematic and sensitive lipidomic approaches, we validated dif ferent tear collection techniques and report the most comprehensive human tear lipidome to date; comprising more than 600 lipid species from 17 major lipid classes. Our study confers novel insights to the compositional details of the existent tear film model, in particular the disputable amphiphilic lipid sublayer constituents, by demonstrating the presence of cholesteryl sulfate, O-Acyl- α -hydroxy fatty acids, and various sphingolipids and phospholipids in tears. The discovery and quantitation of the relative abundance of various tear lipid amphiphiles reported herein are expected to have a profound impact on the current understanding of the existent human tear film model. -Lam, S. M., L. Tong, X. Duan, A. Petznick, M. R. Wenk, and G. Shui. Extensive characterization of human tear fl uid collected using different techniques unravels the presence of novel lipid amphiphiles. Copyright © 2014 by the American Society for Biochemistry and Molecular Biology, Inc..
Source Title: Journal of Lipid Research
URI: http://scholarbank.nus.edu.sg/handle/10635/110534
ISSN: 00222275
DOI: 10.1194/jlr.M044826
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