Please use this identifier to cite or link to this item: https://doi.org/10.1186/1746-6148-8-138
Title: Reproducibility and age-related changes of ocular parametric measurements in rabbits
Authors: Riau, A.K.
Tan, N.Y.S.
Angunawela, R.I.
Htoon, H.M.
Chaurasia, S.S.
Mehta, J.S. 
Keywords: Anterior chamber
Cornea
Keratometry
Rabbit
Refractive
Reproducibility
Issue Date: 19-Aug-2012
Citation: Riau, A.K., Tan, N.Y.S., Angunawela, R.I., Htoon, H.M., Chaurasia, S.S., Mehta, J.S. (2012-08-19). Reproducibility and age-related changes of ocular parametric measurements in rabbits. BMC Veterinary Research 8 : -. ScholarBank@NUS Repository. https://doi.org/10.1186/1746-6148-8-138
Abstract: Background: The rabbit is a common animal model for ophthalmic research, especially corneal research. Ocular structures grow rapidly during the early stages of life. It is unclear when the rabbit cornea becomes mature and stabilized. We investigated the changes of keratometry, refractive state and central corneal thickness (CCT) with age. In addition, we studied the intra- and inter-observer reproducibility of anterior chamber depth (ACD) and anterior chamber width (ACW) measurements in rabbits using anterior segment-optical coherence tomography (AS-OCT).Results: The growth of New Zealand White rabbits (n = 16) were monitored from age 1 to 12 months old. Corneal keratometric and refractive values were obtained using an autorefractor/keratometer, and CCT was measured using an AS-OCT. Keratometry and CCT changed rapidly from 1 to 7 months and appeared to be stabilizing after 8 months. The reduction of corneal curvature was approximately 1.36 diopter (D)/month from age 1 to 7 months, but the change decelerated to 0.30 D/month from age 8 to 12 months. An increase of 10 μm/month in CCT was observed from age 1 to 7 months, but the gain was reduced to less than 1 μm/month from age 8 to 12 months. There was a hyperopic shift over the span of 12 months, albeit the increase in spherical equivalent was slow and gradual. Rabbits of random age were then selected for 2 repeated ACD and ACW measurements by 2 independent and masked observers. Bland-Altman plots revealed a good agreement of ACD and ACW measurements inter- and intra-observer and the ranges of 95% limit of agreement were acceptable from a clinical perspective.Conclusions: Corneal keratometry, spherical equivalent refraction and CCT changed significantly during the first few months of life of rabbits. Young rabbits have been used in a large number of eye research studies. In certain settings, the ocular parametric changes are an important aspect to note as they may alter the findings made in a rabbit experimental model. In this study, we have also demonstrated for the first time a good between observer reproducibility of measurements of ocular parameters in an animal model by using an AS-OCT. © 2012 Riau et al.; licensee BioMed Central Ltd.
Source Title: BMC Veterinary Research
URI: http://scholarbank.nus.edu.sg/handle/10635/110247
ISSN: 17466148
DOI: 10.1186/1746-6148-8-138
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

11
checked on Jun 21, 2018

WEB OF SCIENCETM
Citations

12
checked on Apr 25, 2018

Page view(s)

23
checked on May 4, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.