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Title: | Isolation of a novel chiral phytoestrogen breviflavone B from epimedium herb by a new approach of liquid chromatography | Authors: | Soh, S.F. Tay, J.Y. Li, J. Qian, L. Yin, X. Wei, Q. Yang, H. Wang, L. Wang, D. Yong, E.L. Gong, Y. |
Keywords: | Liquid chromatography Nature product separation ODS-MCR-HPS TCM |
Issue Date: | 2013 | Citation: | Soh, S.F., Tay, J.Y., Li, J., Qian, L., Yin, X., Wei, Q., Yang, H., Wang, L., Wang, D., Yong, E.L., Gong, Y. (2013). Isolation of a novel chiral phytoestrogen breviflavone B from epimedium herb by a new approach of liquid chromatography. Advanced Materials Research 663 : 303-307. ScholarBank@NUS Repository. https://doi.org/10.4028/www.scientific.net/AMR.663.303 | Abstract: | Breviflavone B is a novel chiral phytoestrogen that mimics the biological functions of 17β-estradiol (E2), an estrogen which is naturally produced in our body. It has exhibited a much higher estrogen receptor (ER) bioactivity than E2 or other phytoestrogens. Breviflavone B specifically activates ER at a low dose without activating other steroid receptors, and inhibits estrogen-stimulated breast cancer cell proliferation at high doses, making it a potential treatment option for breast cancer patients. In this report, a new liquid chromatography aprroach has been developed to isolate Breviflavone B from Epimedium Brevicornum herbal extracts. After passing through DIAION HP20 resin, the ethanolic extract of Epimedium Brevicornum was firstly fractionalized by a Diol column, then further separated by a C18 column using preparative HPLC, and finally purified by an ODS-MCR-HPS column in HPLC under gradient mode. In each stage of separation, the ER bioactivities of the each fraction were measured by estrogen receptor-driven reporter gene bioassays to guide the chromatographic fractionations. The results showed that the new combination of Diol and C18 and ODS-MCR-HPS column chromatography provided better separation selectivity than our previously-reported C18 column chromatography for isolation of Breviflavon B from Epemidium herbs. Pure Breviflavon B was successfully isolated from the ethanolic extract of Epimedium Brevicornum herb via this new approach of chromatographic fractionations guided by the bioassays. © (2013) Trans Tech Publications, Switzerland. | Source Title: | Advanced Materials Research | URI: | http://scholarbank.nus.edu.sg/handle/10635/109756 | ISBN: | 9783037856000 | ISSN: | 10226680 | DOI: | 10.4028/www.scientific.net/AMR.663.303 |
Appears in Collections: | Staff Publications |
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