Please use this identifier to cite or link to this item: https://doi.org/10.3233/JAD-2010-1348
Title: Profiling brain and plasma lipids in human apoe ε2, ε3, and ε4 knock - In mice using electrospray ionization mass spectrometry
Authors: Sharman, M.J.
Shui, G. 
Fernandis, A.Z.
Lim, W.L.F.
Berger, T.
Hone, E.
Taddei, K.
Martins, I.J.
Ghiso, J.
Buxbaum, J.D.
Gandy, S.
Wenk, M.R.
Martins, R.N.
Keywords: Alzheimer's disease
APOE genotype
cholesterol
glycerophospholipids
lipidomics
sphingolipids
Issue Date: 2010
Citation: Sharman, M.J., Shui, G., Fernandis, A.Z., Lim, W.L.F., Berger, T., Hone, E., Taddei, K., Martins, I.J., Ghiso, J., Buxbaum, J.D., Gandy, S., Wenk, M.R., Martins, R.N. (2010). Profiling brain and plasma lipids in human apoe ε2, ε3, and ε4 knock - In mice using electrospray ionization mass spectrometry. Journal of Alzheimer's Disease 20 (1) : 105-111. ScholarBank@NUS Repository. https://doi.org/10.3233/JAD-2010-1348
Abstract: It is known that apolipoprotein E (ApoE) is essential for normal lipid metabolism. ApoE is the major apolipoprotein in the central nervous system and plays a key role in neurobiology by mediating the transport of cholesterol, phospholipids, and sulfatides. We therefore examined APOE ε2, ε3, and ε4 knock-in mice, using electrospray ionization mass spectrometry to determine if APOE genotype or age leads to altered levels in the brain of a number of glycerophospholipids (phosphatidylinositol, PI; phosphatidylethanolamine, PE; phosphatidic acid, PA, phosphatidylserine, PS; phosphatidylcholine, PC), sphingolipids (sphingomyelin, SM; ceramide, Cer), cholesterol, and triacylglycerols. We observed slight changes within individual PI, PE, PC, Cer, and SM lipid levels in APOE ε2 and ε4 mice compared to APOE ε3 mice. However, overall, we did not observe any major effects in APOE ε4 knock-in mice for the levels of the glycerophospholipids measured, as compared to APOE ε2 and ε3 mice. Our findings indicate that variations in ApoE isoforms do not per se affect bulk lipid homeostasis in the brain. These findings indicate that APOE ε4 is not associated with disturbances in brain sterol or sphingolipids in the absence of environmental factors. © 2010 - IOS Press and the authors.
Source Title: Journal of Alzheimer's Disease
URI: http://scholarbank.nus.edu.sg/handle/10635/109547
ISSN: 13872877
DOI: 10.3233/JAD-2010-1348
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

15
checked on Sep 19, 2018

WEB OF SCIENCETM
Citations

16
checked on Sep 19, 2018

Page view(s)

45
checked on Sep 14, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.