Please use this identifier to cite or link to this item: https://doi.org/10.1089/scd.2013.0430
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dc.titlePatz1 regulates embryonic stem cell identity
dc.contributor.authorOw, J.R.
dc.contributor.authorMa, H.
dc.contributor.authorJean, A.
dc.contributor.authorGoh, Z.
dc.contributor.authorLee, Y.H.
dc.contributor.authorChong, Y.M.
dc.contributor.authorSoong, R.
dc.contributor.authorFu, X.-Y.
dc.contributor.authorYang, H.
dc.contributor.authorWu, Q.
dc.date.accessioned2014-11-26T07:46:49Z
dc.date.available2014-11-26T07:46:49Z
dc.date.issued2014-05-15
dc.identifier.citationOw, J.R., Ma, H., Jean, A., Goh, Z., Lee, Y.H., Chong, Y.M., Soong, R., Fu, X.-Y., Yang, H., Wu, Q. (2014-05-15). Patz1 regulates embryonic stem cell identity. Stem Cells and Development 23 (10) : 1062-1073. ScholarBank@NUS Repository. https://doi.org/10.1089/scd.2013.0430
dc.identifier.issn15578534
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/109511
dc.description.abstractEmbryonic stem cells (ESCs) derived from the inner cell mass (ICM) of blastocysts are pluripotent. Pluripotency is maintained by a transcriptional network in which Oct4 and Nanog are master regulators. Notably, several zinc finger transcription factors have important roles in this network. Patz1, a BTB/POZ-domain-containing zinc finger protein, is expressed at higher levels in the ICM relative to the trophectoderm. However, its function in pluripotency has been poorly studied. Here, we show that Patz1 is an important regulator of pluripotency in ESCs. Patz1 RNAi, chromatin immunoprecipitation (ChIP), and reporter assays indicate that Patz1 directly regulates Pou5f1 and Nanog. Global transcriptome changes upon Patz1 knockdown largely involve upregulation of apoptotic genes and downregulation of cell cycle and cellular metabolism genes. Patz1 ChIP sequencing further identified more than 5,000 binding sites of Patz1 in mouse genome, from which two binding motifs were extracted. Further, gene ontology analysis of genes associated with the binding sites displays enrichment for proximity to developmental genes. In addition, embryoid body assays suggest that Patz1 represses developmental genes. Together, these results propose that Patz1 is important for ESC pluripotency. © Copyright 2014, Mary Ann Liebert, Inc. 2014.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1089/scd.2013.0430
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1089/scd.2013.0430
dc.description.sourcetitleStem Cells and Development
dc.description.volume23
dc.description.issue10
dc.description.page1062-1073
dc.description.codenSCDTA
dc.identifier.isiut000335756800003
Appears in Collections:Staff Publications

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