Please use this identifier to cite or link to this item: https://doi.org/10.3727/096368910X564085
Title: Characterization of human umbilical cord lining-derived epithelial cells and transplantation potential
Authors: Zhou, Y.
Gan, S.U.
Lin, G.
Lim, Y.T.
Masilamani, J. 
Mustafa, F.B.
Phua, M.L.
Rivino, L.
Phan, T.T.
Lee, K.O.
Calne, R.
MacAry, P.A.
Keywords: Cell transplantation
HLA-G
Immune regulation
Tolerance
Umbilical cord lining cell
Issue Date: 2011
Citation: Zhou, Y., Gan, S.U., Lin, G., Lim, Y.T., Masilamani, J., Mustafa, F.B., Phua, M.L., Rivino, L., Phan, T.T., Lee, K.O., Calne, R., MacAry, P.A. (2011). Characterization of human umbilical cord lining-derived epithelial cells and transplantation potential. Cell Transplantation 20 (11-12) : 1827-1841. ScholarBank@NUS Repository. https://doi.org/10.3727/096368910X564085
Abstract: In this study we describe the derivation and immunological characterization of a primary epithelial cell type from the human umbilical cord membrane. These cord lining epithelial cells (CLECs) expressed and/or secreted isoforms of the nonclassical human leukocyte antigen class I (HLA-1b) glycoproteins, HLA-G and E. Conditioned media from CLECs inhibited mitogen-stimulated T-lymphocyte responses, and in a mixed leukocyte reaction (MLR) assay, cocultured CLECs inhibited allogeneic responses with a concomitant reduction in proinflammatory cytokines. Using a transwell coculture system, it was demonstrated that these immu-noregulatory effects were mediated by soluble factors secreted by CLECs, in a dose-dependent manner. Functional studies using HLA-G blocking antibody showed that the effects of CLEC-secreted products could be inhibited, thus demonstrating a significant and important role for soluble HLA-G. In vivo, we show that transplanted CLECs could be maintained for extended periods in immunocompetent mice where xenorejec-tion rapidly destroyed primary keratinocytes, a control human epithelial cell type. Additionally, CLECs delayed the rejection of keratinocytes and extended their survival when cotransplanted, indicating an ability to protect adjacent human cell types that would otherwise be rejected if transplanted alone. We also show that CLECs transduced with a modified human proinsulin gene were transplanted intraperitoneally into strep-tozotocin (STZ)-induced diabetic mice, resulting in significantly lower levels of serum glucose compared to control mice. This study has characterized the immunological properties of CLECs and tested a potential therapeutic application in the treatment of a type 1 diabetes mouse model. © 2011 Cognizant Comm. Corp.
Source Title: Cell Transplantation
URI: http://scholarbank.nus.edu.sg/handle/10635/109238
ISSN: 09636897
DOI: 10.3727/096368910X564085
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

11
checked on Dec 17, 2018

WEB OF SCIENCETM
Citations

10
checked on Dec 17, 2018

Page view(s)

60
checked on Dec 7, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.