Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.ygyno.2012.06.039
DC Field | Value | |
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dc.title | 14-3-3σ mediates G2-M arrest produced by 5-aza-2′-deoxycytidine and possesses a tumor suppressor role in endometrial carcinoma cells | |
dc.contributor.author | Steiner, M. | |
dc.contributor.author | Clark, B. | |
dc.contributor.author | Tang, J.-Z. | |
dc.contributor.author | Zhu, T. | |
dc.contributor.author | Lobie, P.E. | |
dc.date.accessioned | 2014-11-26T07:42:15Z | |
dc.date.available | 2014-11-26T07:42:15Z | |
dc.date.issued | 2012-10 | |
dc.identifier.citation | Steiner, M., Clark, B., Tang, J.-Z., Zhu, T., Lobie, P.E. (2012-10). 14-3-3σ mediates G2-M arrest produced by 5-aza-2′-deoxycytidine and possesses a tumor suppressor role in endometrial carcinoma cells. Gynecologic Oncology 127 (1) : 231-240. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ygyno.2012.06.039 | |
dc.identifier.issn | 00908258 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/109133 | |
dc.description.abstract | Objectives: To determine the effect of 5-aza-2′-deoxycytidine (DAC) on human endometrial carcinoma cell (HECC) oncogenicity and demonstrate a molecular mechanism by which DAC modulates HECC oncogenicity. Methods: The effect of DAC was tested on HECC RL95-2, AN3, Ishikawa and ECC1 cells. The role of 14-3-3σ on HECC oncogenicity in response to DAC treatment was evaluated in RL95-2 and AN3 cells after forced expression or silencing of 14-3-3σ gene expression. Results: Treatment of HECC with DAC produced non-cytotoxic cell growth inhibition and G2/M cell cycle arrest. This effect was strongly correlated with increased expression of p21 and 14-3-3σ. Silencing of 14-3-3σ induced cellular proliferation and reduced the effect of DAC on cell cycle arrest in G2/M phases. Conversely, forced expression of 14-3-3σ showed the opposite effect. Furthermore, forced expression of 14-3-3σ in human endometrial cell lines reduced cell growth and colony formation. Conclusions: We suggest that 14-3-3σ in HECC suppresses cell proliferation and mediates DAC induced G2/M arrest and inhibition of cell proliferation in HECC. © 2012 Elsevier Inc. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.ygyno.2012.06.039 | |
dc.source | Scopus | |
dc.subject | 14-3-3sigma (stratifin SFN) | |
dc.subject | DAC-5-aza-2′ deoxycytidine | |
dc.subject | Endometrial cancer EC | |
dc.subject | G2-M cell cycle progression inhibition | |
dc.subject | Gene methylation | |
dc.type | Article | |
dc.contributor.department | PHARMACOLOGY | |
dc.description.doi | 10.1016/j.ygyno.2012.06.039 | |
dc.description.sourcetitle | Gynecologic Oncology | |
dc.description.volume | 127 | |
dc.description.issue | 1 | |
dc.description.page | 231-240 | |
dc.description.coden | GYNOA | |
dc.identifier.isiut | 000308783800042 | |
Appears in Collections: | Staff Publications |
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