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Title: In vitro directed differentation of mouse embryonic stem cells into insulin-producing cells
Authors: León-Quinto, T.
Jones, J.
Skoudy, A.
Burcin, M.
Soria, B. 
Keywords: Diabetes
Embryonic stem cells
Exogenous agents
Insulin-producing cells
Islet progenitors
Issue Date: Aug-2004
Citation: León-Quinto, T., Jones, J., Skoudy, A., Burcin, M., Soria, B. (2004-08). In vitro directed differentation of mouse embryonic stem cells into insulin-producing cells. Diabetologia 47 (8) : 1442-1451. ScholarBank@NUS Repository.
Abstract: Aims/hypothesis. We recently demonstrated that insulin-producing cells derived from embryonic stem cells normalise hyperglycaemia in transplanted diabetic mice. The differentiation and selection procedure, however, was successful in less than 5% of the assays performed. Thus, to improve its effectiveness, new strategies have been developed, which increase the number of islet cells or islet progenitors. Methods. Mouse embryonic stem cells transfected with a plasmid containing the Nkx6.1 promoter gene followed by a neomycin-resistance gene, were cultured with factors known to participate in endocrine pancreatic development and factors that modulate signalling pathways involved in these processes. Neomycin was used to select the Nkx6.1-positive cells, which also express insulin. The transfected cells were differentiated using several exogenous agents, followed by selection of Nkx6.1-positive cells. The resulting cells were analysed for pancreatic gene and protein expression by immunocytochemistry, RT-PCR and radioimmunoassay. Also, proliferation assays were performed, as well as transplantation to streptozotocin-induced diabetic mice. Results. The protocols yielded cell cultures with approximately 20% of cells co-expressing insulin and Pdx-1. Cell trapping selection yielded an almost pure population of insulin-positive cells, which expressed the beta cell genes/proteins Pdx-1, Nkx6.1, insulin, glucokinase, GLUT-2 and Sur-1. Subsequent transplantation to streptozotocin-induced diabetic mice normalised their glycaemia during the time period of experimentation, proving the efficiency of the protocols. Conclusions/interpretation. These methods were both highly efficient and very reproducible, resulting in a new strategy to obtain insulin-containing cells from stem cells with a near 100% success rate, while actively promoting the maturation of the exocytotic machinery. © Springer-Verlag 2004.
Source Title: Diabetologia
ISSN: 0012186X
DOI: 10.1007/s00125-004-1458-8
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