Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/108089
Title: Targeting of liposomes via passively-adsorbed monclonal antibodies to human hepatocellular carcinoma cells in vitro
Authors: Tan, L. 
Gregoriadis, G.
Issue Date: 1990
Citation: Tan, L.,Gregoriadis, G. (1990). Targeting of liposomes via passively-adsorbed monclonal antibodies to human hepatocellular carcinoma cells in vitro. Asia Pacific Journal of Pharmacology 5 (3) : 219-222. ScholarBank@NUS Repository.
Abstract: Small unilamellar vesicles (SUV) were coated with monoclonal antibodies RF-HCC1 (IgG1), RF-HCC2 (IgM) and MOPC 21 IgG1, which bind to surface antigens on Mahlavu cells, by passive adsorption. Adsorption values for the three antibodies were 35.1%, 51.6% and 12.2% respectively. On incubation of the liposomes containing the aqueous marker carbocyfluorescein (CF) with Mahlavu cells in vitro at 4°C, it was seen that binding of antibody and association of CF with cells took place in a biphasic manner with maximal binding occuring by 1 hour followed by a plateau phase. These results indicate that passively-adsorbed antibodies represent a rapid, conventient and effective method of ligand-mediated targeting of SUV to cells in vitro.
Source Title: Asia Pacific Journal of Pharmacology
URI: http://scholarbank.nus.edu.sg/handle/10635/108089
ISSN: 02179687
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

43
checked on Oct 5, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.