Please use this identifier to cite or link to this item:
https://doi.org/10.1007/s10495-006-0285-4
DC Field | Value | |
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dc.title | Dose-dependant radiation-induced apoptosis in a cochlear cell-line | |
dc.contributor.author | Low, W.-K. | |
dc.contributor.author | Tan, M.G.K. | |
dc.contributor.author | Sun, L. | |
dc.contributor.author | Chua, A.W.C. | |
dc.contributor.author | Goh, L.-K. | |
dc.contributor.author | Wang, D.-Y. | |
dc.date.accessioned | 2014-11-20T03:16:28Z | |
dc.date.available | 2014-11-20T03:16:28Z | |
dc.date.issued | 2006-12 | |
dc.identifier.citation | Low, W.-K., Tan, M.G.K., Sun, L., Chua, A.W.C., Goh, L.-K., Wang, D.-Y. (2006-12). Dose-dependant radiation-induced apoptosis in a cochlear cell-line. Apoptosis 11 (12) : 2127-2136. ScholarBank@NUS Repository. https://doi.org/10.1007/s10495-006-0285-4 | |
dc.identifier.issn | 13608185 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/107936 | |
dc.description.abstract | Cisplatin and gentamycin are both ototoxic and they have been shown to induce cochlear cell apoptosis. Although radiation is also ototoxic, radiation-induced apoptosis in cochlear cells has not been studied. This study aimed to investigate the biophysical changes of dose-related radiation-induced cochlear cell apoptosis in an experimental model. Post gamma-irradiation apoptosis was demonstrated in the cochlear cell-line OC-k3 by flow cytometry and TUNEL assay. This was dose-dependant with enhanced apoptosis resulting after 20 than 5 Gy, and occurred predominantly at 72 h post-irradiation. Microarray analysis showed associated dose-dependant apoptotic gene regulation changes. Western blotting revealed p53 up-regulation of at 72 h and phosphorylation at 3, 24, 48 and 72 h after irradiation. Early activation of c-jun occurred at 3 h, but was not sustained with time. Associated dose-dependant intracellular generation of reactive oxygen species (ROS) was also demonstrated using 2′, 7′-dichlorofluorescein diacetate. In conclusion, this study demonstrated a dose-dependant cochlear cell apoptosis and associated ROS generation after irradiation, with p53 possibly playing a key role. Based on this ROS-linked apoptotic model, anti-oxidants and anti-apoptotic factors could potentially be used to prevent radiation-induced sensori-neural hearing loss. As these medications can be delivered topically through the middle ear, their systematic side effects could therefore be minimized. © 2006 Springer Science + Business Media, LLC. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/s10495-006-0285-4 | |
dc.source | Scopus | |
dc.subject | Cell death | |
dc.subject | Deafness | |
dc.subject | Hair-cell | |
dc.subject | Hearing loss | |
dc.subject | Ototoxicity | |
dc.subject | Radiotherapy | |
dc.type | Article | |
dc.contributor.department | OTOLARYNGOLOGY | |
dc.description.doi | 10.1007/s10495-006-0285-4 | |
dc.description.sourcetitle | Apoptosis | |
dc.description.volume | 11 | |
dc.description.issue | 12 | |
dc.description.page | 2127-2136 | |
dc.description.coden | APOPF | |
dc.identifier.isiut | 000242417300005 | |
Appears in Collections: | Staff Publications |
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