Please use this identifier to cite or link to this item: https://doi.org/10.1128/JB.186.22.7645-7652.2004
Title: Molecular identification of the urea uptake system and transcriptional analysis of urea transporter- and urease-encoding genes in Corynebacterium glutamicum
Authors: Beckers, G.
Bendt, A.K. 
Krämer, R.
Burkovski, A.
Issue Date: Nov-2004
Citation: Beckers, G., Bendt, A.K., Krämer, R., Burkovski, A. (2004-11). Molecular identification of the urea uptake system and transcriptional analysis of urea transporter- and urease-encoding genes in Corynebacterium glutamicum. Journal of Bacteriology 186 (22) : 7645-7652. ScholarBank@NUS Repository. https://doi.org/10.1128/JB.186.22.7645-7652.2004
Abstract: The molecular identification of the Corynebacterium glutamicum urea uptake system is described. This ABC-type transporter is encoded by the urtABCDE operon, which is transcribed in response to nitrogen limitation. Expression of the urt genes is regulated by the global nitrogen regulator AmtR, and an amtR deletion strain showed constitutive expression of the urtABCDE genes. The AmtR repressor protein also controls transcription of the urease-encoding ureABCEFGD genes in C. glutamicum. The are gene cluster forms an operon which is mainly transcribed in response to nitrogen starvation. To confirm the increased synthesis of urease subunits under nitrogen limitation, proteome analyses of cytoplasmic protein extracts from cells grown under nitrogen surplus and nitrogen limitation were carried out, and five of the seven urease subunits were identified.
Source Title: Journal of Bacteriology
URI: http://scholarbank.nus.edu.sg/handle/10635/107783
ISSN: 00219193
DOI: 10.1128/JB.186.22.7645-7652.2004
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