Please use this identifier to cite or link to this item: https://doi.org/10.1128/AAC.00440-06
Title: Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: Identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b
Authors: Kumar, A.
Chua, K.-L. 
Schweizer, H.P.
Issue Date: Oct-2006
Source: Kumar, A., Chua, K.-L., Schweizer, H.P. (2006-10). Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: Identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b. Antimicrobial Agents and Chemotherapy 50 (10) : 3460-3463. ScholarBank@NUS Repository. https://doi.org/10.1128/AAC.00440-06
Abstract: Construction and integration of recombinant mini-Tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and biosafe Pseudomonas aeruginosa host was validated as a generally applicable method for studies of uncharacterized bacterial efflux pumps. Using this method, the Burkholderia pseudomallei bpeEF-oprC operon was shown to encode a chloramphenicol and trimethoprim efflux pump. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Source Title: Antimicrobial Agents and Chemotherapy
URI: http://scholarbank.nus.edu.sg/handle/10635/107726
ISSN: 00664804
DOI: 10.1128/AAC.00440-06
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