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|Title:||Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: Identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b|
|Source:||Kumar, A., Chua, K.-L., Schweizer, H.P. (2006-10). Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: Identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b. Antimicrobial Agents and Chemotherapy 50 (10) : 3460-3463. ScholarBank@NUS Repository. https://doi.org/10.1128/AAC.00440-06|
|Abstract:||Construction and integration of recombinant mini-Tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and biosafe Pseudomonas aeruginosa host was validated as a generally applicable method for studies of uncharacterized bacterial efflux pumps. Using this method, the Burkholderia pseudomallei bpeEF-oprC operon was shown to encode a chloramphenicol and trimethoprim efflux pump. Copyright © 2006, American Society for Microbiology. All Rights Reserved.|
|Source Title:||Antimicrobial Agents and Chemotherapy|
|Appears in Collections:||Staff Publications|
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