Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/107652
Title: Molecular analysis of the plasmid-borne bed gene cluster from Pseudomonas putida ML2 and cloning of the cis-benzene dihydrodiol dehydrogenase gene
Authors: Tan, H.-M. 
Fong, K.P.-Y.
Keywords: catabolic plasmid
dehydrogenase
dioxygenase
Pseudomonas putida
Issue Date: 1993
Source: Tan, H.-M.,Fong, K.P.-Y. (1993). Molecular analysis of the plasmid-borne bed gene cluster from Pseudomonas putida ML2 and cloning of the cis-benzene dihydrodiol dehydrogenase gene. Canadian Journal of Microbiology 39 (4) : 357-362. ScholarBank@NUS Repository.
Abstract: Pseudomonas putida ML2 contains a large catabolic plasmid, pHMT112, carrying genes that encode the dioxygenase and dehydrogenase involved in the catabolism of benzene via the ortho or β-ketoadipate pathway. pHMT112 was derived from a larger and less stable plasmid in P. putida ML2 following growth on succinate as carbon and energy source but was, however, stably maintained in P. putida even in the absence of selection for growth on benzene. Cleavage sites for the restriction endonucleases DraI, XbaI, and BamHI were mapped on the plasmid. A region of the plasmid, downstream of the benzene dioxygenase genes (bedC1C2BA), was found to encode the cis-benzene dihydrodiol dehydrogenase gene (bedB). Recombinant Escherichia coli containing bedC1C2BAD genes was found to express benzene dioxygenase and dehydrogenase activity, indicated by the production of catechol when incubated in the presence of benzene. Hybridization using benzene dioxygenase genes as probes was used to construct a restriction map of the 35.5-kb XhoI-DraI fragment on which the bed operon was located.
Source Title: Canadian Journal of Microbiology
URI: http://scholarbank.nus.edu.sg/handle/10635/107652
ISSN: 00084166
Appears in Collections:Staff Publications

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