Please use this identifier to cite or link to this item:
|Title:||The first cell cycle after transfer of somatic cell nuclei in a non-human primate|
|Authors:||Ng, S.-C. |
Geok Tan, L.
Somatic cell nuclear transfer
|Source:||Ng, S.-C., Chen, N., Yip, W.-Y., Liow, S.-L., Tong, G.-Q., Martelli, B., Geok Tan, L., Martelli, P. (2004-05). The first cell cycle after transfer of somatic cell nuclei in a non-human primate. Development 131 (10) : 2475-2484. ScholarBank@NUS Repository. https://doi.org/10.1242/dev.01118|
|Abstract:||Production of genetically identical non-human primates through somatic cell nuclear transfer (SCNT) can provide diseased genotypes for research and clarify embryonic stem cell potentials. Understanding the cellular and molecular changes in SCNT is crucial to its success. Thus the changes in the first cell cycle of reconstructed zygotes after nuclear transfer (NT) of somatic cells in the Long-tailed Macaque (Macaca fascicularis) were studied. Embryos were reconstructed by injecting cumulus and fibroblasts from M. fascicularis and M. silenus, into enucleated M. fascicularis oocytes. A spindle of unduplicated premature condensed chromosome (PCC spindle) from the donor somatic cell was formed at 2 hours after NT. Following activation, the chromosomes segregated and moved towards the two PCC spindle poles, then formed two nuclei. Twenty-four hours after activation, the first cell division occurred. A schematic of the first cell cycle changes following injection of a somatic cell into an enucleated oocyte is proposed. Ninety-three reconstructed embryos were transferred into 31 recipients, resulting in 7 pregnancies that were confirmed by ultrasound; unfortunately none progressed beyond 60 days.|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 6, 2017
WEB OF SCIENCETM
checked on Nov 21, 2017
checked on Dec 10, 2017
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.