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Title: Embryonic stem cell lines from human blastocysts: Somatic differentiation in vitro
Authors: Reubinoff, B.E.
Pera, M.F.
Fong, C.-Y. 
Trounson, A.
Bongso, A. 
Keywords: Differentiation
Embryonic stem cell
Neural progenitor
Issue Date: 2000
Source: Reubinoff, B.E.,Pera, M.F.,Fong, C.-Y.,Trounson, A.,Bongso, A. (2000). Embryonic stem cell lines from human blastocysts: Somatic differentiation in vitro. Nature Biotechnology 18 (4) : 399-404. ScholarBank@NUS Repository.
Abstract: We describe the derivation of pluripotent embryonic stem (ES) cells from human blastocysts. Two diploid ES cell lines have been cultivated in vitro for extended periods while maintaining expression of markers characteristic of pluripotent primate cells. Human ES cells express the transcription factor Oct-4, essential for development of pluripotential cells in the mouse. When grafted into SCID mice, both lines give rise to teratomas containing derivatives of all three embryonic germ layers. Both cell lines differentiate in vitro into extraembryonic and somatic cell lineages. Neural progenitor cells may be isolated from differentiating ES cell cultures and induced to form mature neurons. Embryonic stem cells provide a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Source Title: Nature Biotechnology
ISSN: 10870156
DOI: 10.1038/74447
Appears in Collections:Staff Publications

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