Please use this identifier to cite or link to this item: https://doi.org/10.1023/A:1022527714175
Title: Destruction of protamine in human sperm inhibits sperm binding and penetration in the zona-free hamster penetration test but increases sperm head decondensation and male pronuclear formation in the hamster-ICSI assay
Authors: Ahmadi, A.
Ng, S.-C. 
Keywords: Binding
Dead sperm
Male pronuclear formation
Penetration
Protamine-damaged
Sperm head decondensation
Issue Date: 1999
Source: Ahmadi, A., Ng, S.-C. (1999). Destruction of protamine in human sperm inhibits sperm binding and penetration in the zona-free hamster penetration test but increases sperm head decondensation and male pronuclear formation in the hamster-ICSI assay. Journal of Assisted Reproduction and Genetics 16 (3) : 128-132. ScholarBank@NUS Repository. https://doi.org/10.1023/A:1022527714175
Abstract: Purpose: Our purpose was to investigate the fertilizing ability of human protamine-damaged sperm in a heterologous system using hamster oocytes. Methods: The protamine of the sperm were damaged by exposure to dithiothreitol, a disulfide-reducing agent. Their ability to penetrate and form male pronuclei were investigated using the zona-free hamster penetration test and the hamster-intracytoplasmic sperm injection assay respectively. Results: The zona-free hamster penetration test revealed that protamine- damaged sperm are unable to bind and penetrate the hamster oocyte. On the other hand, hamster-intracytoplasmic sperm injection assay results showed that 56.9% and 39.2% of the injected oocytes developed male pronuclei in protamine-damaged and live-intact sperm groups, respectively with a significant difference in these rates (P < 0.01). Conclusions: This study shows that protamine-damaged sperm are able to undergo sperm head decondensation and male pronuclear formation only when injected into the ooplasm, although they cannot bind and penetrate through the zona and enter the ooplasm.
Source Title: Journal of Assisted Reproduction and Genetics
URI: http://scholarbank.nus.edu.sg/handle/10635/107601
ISSN: 10580468
DOI: 10.1023/A:1022527714175
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