Please use this identifier to cite or link to this item: https://doi.org/10.1093/humrep/14.9.2279
Title: Developmental capacity of damaged spermatozoa
Authors: Ahmadi, A.
Ng, S.-C. 
Keywords: Apoptosis
Cytosolic sperm factor
DNA damage
Necrosis
Oocyte activation
Issue Date: 1999
Citation: Ahmadi, A., Ng, S.-C. (1999). Developmental capacity of damaged spermatozoa. Human Reproduction 14 (9) : 2279-2285. ScholarBank@NUS Repository. https://doi.org/10.1093/humrep/14.9.2279
Abstract: We report the first detailed and systematic study in a mammalian system to unravel the mystery of the beginnings of life. The fertilizing ability of damaged spermatozoa at various levels of disintegration (cellular and molecular) has been investigated in homologous (mouse) and heterologous (human spermatozoon, hamster oocyte) models. Live pups were produced after destruction of spermatozoa at various cellular and molecular levels followed by injection into oocytes. We demonstrate that with damaged spermatozoa, the key point in the fertilization process is the activation of the oocyte by injection of cytosolic sperm factor. A similar fertilization rate as that using live intact spermatozoa can be achieved following activation. However, the integrity of the genetic material influenced in-vitro development of the embryos and live fetuses. This study contributes to a better understanding of the fertilizing ability of damaged spermatozoa. These findings can be applied clinically to patients with necrozoospermia or very severe oligozoospermia and in wildlife research where damaged spermatozoa from rare species can be used to regenerate young, and hence propagate the species. Also implied is the possible contribution of sperm DNA strand breakage to early pregnancy loss.
Source Title: Human Reproduction
URI: http://scholarbank.nus.edu.sg/handle/10635/107600
ISSN: 02681161
DOI: 10.1093/humrep/14.9.2279
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