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|Title:||Fertilization and development of mouse oocytes injected with membrane-damaged spermatozoa|
Single sperm curling (SSC) test
|Citation:||Ahmadi, A.H., Ng, S.-C. (1997). Fertilization and development of mouse oocytes injected with membrane-damaged spermatozoa. Human Reproduction 12 (12) : 2797-2801. ScholarBank@NUS Repository.|
|Abstract:||The objective of this study was to investigate the influence of sperm plasma membrane on fertilization and development in a mouse model. The sperm plasma membrane was destroyed by exposure to Triton X-100 prior to intracytoplasmic sperm injection (ICSI). A single sperm curling (SSC) test was used to evaluate cell viability. The fertilization rates of oocytes obtained following ICSI of membrane-damaged sperm was not significantly higher than that of the control group (62.4 versus 59.6%). Rates of development to blastocyst were also not significantly different (51.7 and 50%). Inner cell mass (ICM) and total embryo cell numbers in the two groups were not statistically different (16 ± 3.7 versus 14.73 ± 3.35 and 45.8 ± 12.5 versus 39.5 ± 12 respectively). There were no differences in the implantation and live fetus rates between the two groups after transfer to pseudopregnant mice (61.5 and 35.9% respectively for the membrane-damaged group and 53.5 and 31.4% for the intact group). In conclusion, the present study clearly shows that destruction of the sperm plasma membrane does not affect fertilization and further development following injection of membrane-damaged spermatozoa into mouse oocytes. Fertilization and development can be achieved by dead spermatozoa at an early stage of necrosis when only the plasma membrane has been damaged.|
|Source Title:||Human Reproduction|
|Appears in Collections:||Staff Publications|
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