Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/107594
Title: Intracytoplasmic injection of human sperm into frozen-thawed hamster oocytes
Authors: Ahmadi, A.
Bongso, A. 
Ng, S.C. 
Keywords: Cryopreservation
Hamster-ICSI assay
Intracytoplasmic sperm injection (ICSI)
Oocyte
Sperm
Issue Date: 1996
Source: Ahmadi, A.,Bongso, A.,Ng, S.C. (1996). Intracytoplasmic injection of human sperm into frozen-thawed hamster oocytes. Medical Science Research 24 (10) : 693-694. ScholarBank@NUS Repository.
Abstract: Superovulated hamster oocytes were frozen by 2 M DMSO. 89% (499/561) of the oocytes survived freezing and thawing. A hamster-ICSI assay was performed to evaluate the ability of frozen-thawed oocytes to develop male pronuclei. 46% (58/127) of the oocytes had male pronuclear formation following injection of single human sperm into the cytoplasm of frozen-thawed oocytes. There was no significant difference in male pronuclear formation as compared with fresh oocytes. This study shows that hamster oocytes conserved their ability to support human sperm head decondensation and male pronuclear formation following freezing-thawing.
Source Title: Medical Science Research
URI: http://scholarbank.nus.edu.sg/handle/10635/107594
ISSN: 02698951
Appears in Collections:Staff Publications

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