Please use this identifier to cite or link to this item:
|Title:||Immunogenic Potential of Enterococcus faecalis Biofilm under Simulated Growth Conditions|
|Citation:||Mathew, S., Yaw-Chyn, L., Kishen, A. (2010-05). Immunogenic Potential of Enterococcus faecalis Biofilm under Simulated Growth Conditions. Journal of Endodontics 36 (5) : 832-836. ScholarBank@NUS Repository. https://doi.org/10.1016/j.joen.2010.02.022|
|Abstract:||Introduction: The aim of this study was to evaluate the effect of growth modes (planktonic and biofilms) of Enterococcus faecalis on the intracellular survival and ability to produce tumor necrosis factor α (TNF-α) and interleukin (IL)-6 after interacting with monocytes/in vitro-differentiated macrophages. Methods: In vitro biofilms of three E. faecalis strains (ATCC-29212, OG1RF, and FA2-2) were grown on dentin under simulated nutrient-rich and nutrient-deprived conditions. Biofilm-derived E. faecalis cells were incubated with monocytes/in vitro-differentiated human macrophages. A fluorometric assay was used to quantify the surface adherent bacteria, whereas an antibiotic protection assay was used to quantify the internalized E. faecalis cells 6 to 48 hours after interaction with macrophages. TNF-α and IL-6 produced during this interaction were quantified by an enzyme-linked immunosorbent assay. Results: The surface adherence and intracellular survival of E. faecalis within macrophages was significantly higher in biofilm bacteria when compared with planktonic cells (p ≤ 0.05). E. faecalis strains survived within the macrophages for up to 48 hours and produced higher levels of IL-6 with planktonic cells when compared with biofilm cells. Except in FA2-2, the planktonic cells of other strains did not show a significant increase in TNF-α compared with the biofilm cells (p > 0.05). Conclusion: Biofilm cells of E. faecalis interacting with macrophages showed higher potential for surface adherence, intracellular survival and produced lesser amounts of IL-6 and TNF-α when compared to planktonic cells. Further experiments are required to understand the clinical implication of the intracellular survival of E. faecalis biofilm. © 2010 American Association of Endodontists.|
|Source Title:||Journal of Endodontics|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 14, 2018
WEB OF SCIENCETM
checked on Jun 20, 2018
checked on Jun 29, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.