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Title: Annexin-1 regulates growth arrest induced by high levels of estrogen in MCF-7 breast cancer cells
Authors: Ang, E.Z.-F.
Nguyen, H.T.
Sim, H.-L.
Putti, T.C. 
Lim, L.H.K. 
Issue Date: 1-Feb-2009
Citation: Ang, E.Z.-F., Nguyen, H.T., Sim, H.-L., Putti, T.C., Lim, L.H.K. (2009-02-01). Annexin-1 regulates growth arrest induced by high levels of estrogen in MCF-7 breast cancer cells. Molecular Cancer Research 7 (2) : 266-274. ScholarBank@NUS Repository.
Abstract: Estrogen, a naturally occurring female steroid growth hormone, has been implicated as a major risk factor for the development of breast cancer. Recent research into this disease has also correlated Annexin-1 (ANXA1), a glucocorticoid-inducible protein, with the development of breast tumorigenesis. ANXA1 is lost in many cancers, including breast cancer, and this may result in a functional promotion of tumor growth. In this study, we investigated the expression of ANXA1 in MCF-7 cells treated with estrogen and the regulation of estrogen functions by ANXA1. Exposure of MCF-7 breast cancer cells to high physiologic levels (up to 100 nmol/L) of estrogen leads to an up-regulation of ANXA1 expression partially through the activation of cyclic AMP-responsive element binding protein and dependency on activation of the estrogen receptor. In addition, treatment of MCF-7 cells with physiologic levels of estrogen (1 nmol/L) induced proliferation, whereas high pregnancy levels of estrogen (100 nmol/L) induced a growth arrest of MCF-7 cells, associated with constitutive activation of extracellular signal-regulated kinase 1/2 and up-regulation of cell cycle arrest proteins such as p21 waf/cip. Silencing of ANXA1 with specific small interfering RNA reverses the estrogen-dependent proliferation as well as growth arrest and concomitantly modulates extracellular signal-regulated kinase 1/2 phosphorylation. We confirm that ANXA1 is lost in clinical breast cancer, indicating that the antiproliferative protective function of ANXA1 against high levels of estrogen may be lost. Finally, we show that ANXA1-deficient mice exhibit faster carcinogen-induced tumor growth. Our data suggest that ANXA1 may act as a tumor suppressor gene and modulate the proliferative functions of estrogens. Copyright © 2009 American Association for Cancer Research.
Source Title: Molecular Cancer Research
ISSN: 15417786
DOI: 10.1158/1541-7786.MCR-08-0147
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