Please use this identifier to cite or link to this item: https://doi.org/10.1021/ac049351c
Title: Multiplexed hybridization detection of quantum dot-conjugated DNA sequence using surface plasmon enhanced fluorescence microscopy and spectrometry
Authors: Robelek, R.
Niu, L. 
Schmid, E.L.
Knoll, W. 
Issue Date: 15-Oct-2004
Citation: Robelek, R., Niu, L., Schmid, E.L., Knoll, W. (2004-10-15). Multiplexed hybridization detection of quantum dot-conjugated DNA sequence using surface plasmon enhanced fluorescence microscopy and spectrometry. Analytical Chemistry 76 (20) : 6160-6165. ScholarBank@NUS Repository. https://doi.org/10.1021/ac049351c
Abstract: In this study, the general suitability of quantum dot (QD)-DNA conjugates for the surface plasmon enhanced fluorescence spectroscopy technique is demonstrated. Furthermore, the QD-DNA system is transferred to the platform of surface plasmon enhanced fluorescence microscopy. Using this technique together with a microarray format, in which the sensor-bound single-stranded catcher probes are organized in individual surface spots, results in a simultaneous qualitative analysis of QD-conjugated analyte DNA strands as multicolor images. A clear decomposition of different QDx-DNAy mixtures can be achieved for sequential, as well as mixture injections. Besides this, the study describes the successful approach of measuring spectrally resolved surface plasmon enhanced fluorescence signals derived from catcher probe hybridized QD-DNA conjugates.
Source Title: Analytical Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/107134
ISSN: 00032700
DOI: 10.1021/ac049351c
Appears in Collections:Staff Publications

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