Please use this identifier to cite or link to this item:
Title: Two forms of Factor C from the amoebocytes of Carcinoscorpius rotundicauda: purification and characterisation
Authors: Ding, J.L. 
Navas III, M.A.A.
Ho, B.
Keywords: (C. rotundicauda)
Factor C
Protein characterization
Protein purification
Issue Date: 3-Sep-1993
Citation: Ding, J.L.,Navas III, M.A.A.,Ho, B. (1993-09-03). Two forms of Factor C from the amoebocytes of Carcinoscorpius rotundicauda: purification and characterisation. Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular 1202 (1) : 149-156. ScholarBank@NUS Repository.
Abstract: The two apparent forms of the endotoxin-sensitive Factor C which were found to exist in the amoebocytes of horseshoe crabs have been separately purified to homogeneity from the lysate of the South-East Asian species, Carcinoscorpius rotundicauda. Both forms are serine proteinase zymogens having an apparent molecular mass of 132 kDa. By reducing SDS-PAGE, one was shown to consist of a single polypeptide while the other has a heavy chain (80 kDa) and a light chain (52 kDa) bridged by disulfide linkage(s). Both zymogen forms have endotoxin (lipopolysaccharide) receptors to which endotoxin binds to activate their catalytic sites. However, single-chain Factor C appears to have higher-affinity endotoxin-bindings sites which are competitively but reversibly occupied by DMSO when the latter was added during its purification. Another salient difference between the two forms of Factor C is exhibited in their manner of activation by endotoxin. While double-chain Factor C appears similar to that of Tachypleus tridentatus, single-chain Factor C did not undergo any proteolytic cleavage upon activation. This conformational transition of zymogen activation suggests an alternative reversible pathway of endotoxin activation for the single-chain Factor C. © 1993.
Source Title: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
ISSN: 01674838
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

checked on Oct 5, 2018

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.