Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/106811
DC Field | Value | |
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dc.title | Evidence for the involvement of a nuclear NF-κB inhibitor in global down-regulation of the major histocompatibility complex class I enhancer in adenovirus type 12-transformed cells | |
dc.contributor.author | Liu, X. | |
dc.contributor.author | Ge, R. | |
dc.contributor.author | Ricciardi, R.P. | |
dc.date.accessioned | 2014-10-29T07:54:15Z | |
dc.date.available | 2014-10-29T07:54:15Z | |
dc.date.issued | 1996-01 | |
dc.identifier.citation | Liu, X.,Ge, R.,Ricciardi, R.P. (1996-01). Evidence for the involvement of a nuclear NF-κB inhibitor in global down-regulation of the major histocompatibility complex class I enhancer in adenovirus type 12-transformed cells. Molecular and Cellular Biology 16 (1) : 398-404. ScholarBank@NUS Repository. | |
dc.identifier.issn | 02707306 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/106811 | |
dc.description.abstract | Diminished expression of major histocompatibility complex class I antigens on the surface of adenovirus type 12 (Ad12)-transformed cells contributes to their high tumorigenic potential by enabling them to escape immune recognition by cytotoxic T lymphocytes. This low class I antigen expression is due to a block in class I transcription, which is mediated by Ad12 E1A. Genetic analysis has shown that the class I enhancer is the target for transcriptional down-regulation. In this study, we show that the ability of the R1 element of the class I enhancer to stimulate transcription is greatly reduced in Ad12-transformed cells. The loss of functional activity by the R1 element was attributed to loss of binding by the NF-κB p50-p65 heterodimer. NF-κB binding appears to be blocked within the nucleus rather than at the level of nuclear translocation. Significantly, NF-κB binding activity could be recovered from the nuclear extracts of Ad12-transformed cells following detergent treatment, suggesting that the block is mediated through a nuclear inhibitor present in the Ad12-transformed cells. These results, taken together with the fact that the R2 element of the class I enhancer exhibits strong binding to the transcriptional repressor COUP-TF, suggest that the class I enhancer is globally down-regulated in Ad12-transformed cells. | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | ZOOLOGY | |
dc.description.sourcetitle | Molecular and Cellular Biology | |
dc.description.volume | 16 | |
dc.description.issue | 1 | |
dc.description.page | 398-404 | |
dc.description.coden | MCEBD | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Staff Publications |
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