Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.ijpharm.2010.08.023
Title: Wheat germ agglutinin-conjugated PLGA nanoparticles for enhanced intracellular delivery of paclitaxel to colon cancer cells
Authors: Wang, C.
Ho, P.C. 
Lim, L.Y.
Keywords: Colon cancer
Intracellular delivery
Paclitaxel
PLGA nanoparticles
Wheat germ agglutinin (WGA)
Issue Date: 15-Nov-2010
Source: Wang, C., Ho, P.C., Lim, L.Y. (2010-11-15). Wheat germ agglutinin-conjugated PLGA nanoparticles for enhanced intracellular delivery of paclitaxel to colon cancer cells. International Journal of Pharmaceutics 400 (1-2) : 201-210. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ijpharm.2010.08.023
Abstract: The purpose of this study was to investigate the potentiation of the anticancer activity and enhanced cellular retention of paclitaxel-loaded PLGA nanoparticles after surface conjugation with wheat germ agglutinin (WGA) against colon cancer cells. Glycosylation patterns of representative colon cancer cells confirmed the higher expression levels of WGA-binding glycoproteins in the Caco-2 and HT-29 cells, than in the CCD-18Co cells. Cellular uptake and in vitro cytotoxicity of WNP (final formulation) against colon cell lines was evaluated alongside control formulations. Confocal microscopy and quantitative analysis of intracellular paclitaxel were used to monitor the endocytosis and retention of nanoparticles inside the cells. WNP showed enhanced anti-proliferative activity against Caco-2 and HT-29 cells compared to corresponding nanoparticles without WGA conjugation (PNP). The greater efficacy of WNP was associated with higher cellular uptake and sustained intracellular retention of paclitaxel, which in turn was attributed to the over-expression of N-acetyl-d-glucosamine-containing glycoprotein on the colon cell membrane. WNP also demonstrated increased intracellular retention in the Caco-2 (30% of uptake) and HT-29 (40% of uptake) cells, following post-uptake incubation with fresh medium, compared to the unconjugated PNP nanoparticles (18% in Caco-2) and (27% in HT-29), respectively. Cellular trafficking study of WNP showed endocytosed WNP could successful escape from the endo-lysosome compartment and release into the cytosol with increasing incubation time. It may be concluded that WNP has the potential to be applied as a targeted delivery platform for paclitaxel in the treatment of colon cancer. © 2010 Elsevier B.V.
Source Title: International Journal of Pharmaceutics
URI: http://scholarbank.nus.edu.sg/handle/10635/106513
ISSN: 03785173
DOI: 10.1016/j.ijpharm.2010.08.023
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

34
checked on Feb 15, 2018

WEB OF SCIENCETM
Citations

31
checked on Feb 6, 2018

Page view(s)

31
checked on Feb 20, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.