Please use this identifier to cite or link to this item:
Title: Transport of angiotensin peptides across the Caco-2 monolayer
Authors: Chua, H.-L.
Jois, S. 
Sim, M.-K.
Go, M.-L. 
Keywords: Angiotensin peptides
Caco-2 monolayer
des-Asp-angiotensin I
Mechanisms of transport
Solution conformation
Structure-permeability correlations
Issue Date: Aug-2004
Citation: Chua, H.-L., Jois, S., Sim, M.-K., Go, M.-L. (2004-08). Transport of angiotensin peptides across the Caco-2 monolayer. Peptides 25 (8) : 1327-1338. ScholarBank@NUS Repository.
Abstract: The bidirectional transport of the angiotensin peptides - des-Asp-angiotensin I (DAAI), angiotensins III and IV - were studied using human intestinal Caco-2 monolayers. The peptides had low permeability rates but were relatively stable to enzymatic hydrolysis. DAAI was transported by diffusion while angiotensins III and IV were transported by an energy requiring, carrier-mediated process. The physicochemical properties and solution conformations of the peptides were investigated in an attempt to establish structure-transport correlations. Among the three peptides, DAAI was the most hydrophobic, had the highest hydrogen bonding potential and was the only peptide to have a random solution conformation, as determined from circular dichroism, two-dimensional 1H NMR and molecular modelling. On the other hand, the more hydrophilic angiotensin IV had less hydrogen bonding potential and a solution conformation characterized by a β turn. These factors may influence the transport characteristics of DAAI and angiotensin IV. © 2004 Elsevier Inc. All rights reserved.
Source Title: Peptides
ISSN: 01969781
DOI: 10.1016/j.peptides.2004.06.009
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Oct 15, 2018


checked on Oct 8, 2018

Page view(s)

checked on Oct 12, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.