Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2010.11.070
Title: The role of PEG architecture and molecular weight in the gene transfection performance of PEGylated poly(dimethylaminoethyl methacrylate) based cationic polymers
Authors: Venkataraman, S.
Ong, W.L.
Ong, Z.Y.
Joachim Loo, S.C.
Rachel Ee, P.L. 
Yang, Y.Y.
Keywords: Cationic polymers
Gene transfection
Macromolecular architecture
PEGylation
Reversible addition-fragmentation chain transfer (RAFT) polymerization
Issue Date: Mar-2011
Citation: Venkataraman, S., Ong, W.L., Ong, Z.Y., Joachim Loo, S.C., Rachel Ee, P.L., Yang, Y.Y. (2011-03). The role of PEG architecture and molecular weight in the gene transfection performance of PEGylated poly(dimethylaminoethyl methacrylate) based cationic polymers. Biomaterials 32 (9) : 2369-2378. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2010.11.070
Abstract: In this study, we report the synthesis of well-defined model PEGylated poly(dimethylaminoethyl methacrylate) based cationic polymers composed of different PEG architecture with controlled PEG and nitrogen content via reversible addition-fragmentation chain transfer (RAFT) polymerization, and study the effects of PEG architecture and polymer molecular weight on gene delivery and cytotoxicity. Investigation of the physico-chemical interactions of these model cationic polymers with DNA demonstrated that all these polymers effectively complexed with DNA, and PEG topology did not significantly affect the abilities of the polymers to complex and release DNA. However the size and zeta potential of the complexes were found to be influenced by PEG architecture. The polymers with the block-like configurations formed nanosized DNA complexes. In contrast, considerably higher molecular weight was necessary for the copolymer with the statistical configuration of short PEG chains to form such a small complex. Cell line-dependent influence of PEG architecture on cellular uptake, gene expression efficiency and cell viability of the polymer-DNA complexes was observed. The diblock copolymer-DNA complexes induced higher gene expression than the brush-like block copolymer-DNA complexes, and the statistical copolymer-DNA complexes mediated much lower gene expression than the block-like copolymers-DNA complexes. Increasing the molecular weight of statistical polymer to some extent improved gene expression efficiency. The statistical copolymer was less cytotoxic as compared to the block-like copolymers. These findings provide important insights into the effect of PEGylation nature on gene expression, which will be useful for the design of PEGylated gene delivery polymers. © 2010 Elsevier Ltd.
Source Title: Biomaterials
URI: http://scholarbank.nus.edu.sg/handle/10635/106448
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2010.11.070
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

53
checked on May 26, 2018

WEB OF SCIENCETM
Citations

52
checked on Apr 3, 2018

Page view(s)

43
checked on May 25, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.