Please use this identifier to cite or link to this item:
|Title:||Structure-activity and structure-binding studies of des-Asp1-angiotensin I analogues on the rabbit pulmonary artery|
Rabbit pulmonary artery
|Citation:||Chen, W.-S., Sim, M.-K., Go, M.-L. (2002-06-15). Structure-activity and structure-binding studies of des-Asp1-angiotensin I analogues on the rabbit pulmonary artery. Regulatory Peptides 106 (1-3) : 39-46. ScholarBank@NUS Repository. https://doi.org/10.1016/S0167-0115(02)00030-7|
|Abstract:||Structural modification of des-aspartate-angiotensin I (DAA-I), a pharmacologically active peptide, affected its actions on the precontracted cardiac and pulmonary sections of the rabbit pulmonary artery. The displacement of [125I]-Sar1-Ile8-angiotensin II by the DAA-I analogues from membrane homogenates of the whole pulmonary artery was also markedly reduced. Analogues that retained similar responses as DAA-I in the functional assays exhibited binding affinities of similar magnitude as DAA-I. Analogues that had no effect in the functional assay showed markedly reduced binding affinities. The first and fifth positions on DAA-I were identified as critical positions for activity as the replacement of Arg2 and His6 at these positions with alanine completely abolished activity and sharply reduced binding affinities. In contrast, the last two N-terminal amino acids of DAA-I can be modified substantially (D-amino acid and alanine substitution) without loss of activity or binding affinity. The identification of critical and noncritical amino acids would offer useful leads in the design of specific DAA-I antagonists. © 2002 Elsevier Science B.V. All rights reserved.|
|Source Title:||Regulatory Peptides|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on May 26, 2018
WEB OF SCIENCETM
checked on Apr 3, 2018
checked on May 25, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.