Please use this identifier to cite or link to this item: https://doi.org/10.3892/ijo.2013.1843
Title: Role of oxidative stress, endoplasmic reticulum stress and ERK activation in triptolide-induced apoptosis
Authors: Tan, B.-J. 
Chiu, G.N.C. 
Keywords: Apoptosis
Breast cancer
Endoplasmic reticulum stress
ERK
Oxidative stress
Triptolide
Issue Date: May-2013
Source: Tan, B.-J., Chiu, G.N.C. (2013-05). Role of oxidative stress, endoplasmic reticulum stress and ERK activation in triptolide-induced apoptosis. International Journal of Oncology 42 (5) : 1605-1612. ScholarBank@NUS Repository. https://doi.org/10.3892/ijo.2013.1843
Abstract: Since its isolation from Tripterygium wilfordii in 1972, triptolide has been shown to possess potent anticancer activity against a variety of cancers, and has entered phase I clinical trial. It is a diterpenoid triepoxide that acts through multiple molecular targets and signaling pathways. The mitogen-activated protein kinases are well known for their modulation of cell survival and proliferation. In particular, the ERK pathway has a dual role in cell proliferation and cell death. Thus far, data on the effect of triptolide on ERK signaling remain limited. In our current study, we have shown for the first time that ERK activation rather than inhibition occurred in a dose- and time-dependent manner following triptolide treatment in MDA-MB-231 breast cancer cells. ERK activation was crucial in mediating triptolide-induced caspase-dependent apoptosis. Tritpolide-induced ERK activation modulated the expression of the Bcl-2 protein family member Bax but was not involved in the downregulation of Bcl-xL expression. Signals acted upstream of ERK activation included generation of reactive oxygen species (ROS) and endoplasmic reticulum stress predominantly via the PERK eIF2α pathway, as the MEK inhibitor U0126 did not inhibit the phosphorylation of PERK and eIF2α or the generation of ROS.
Source Title: International Journal of Oncology
URI: http://scholarbank.nus.edu.sg/handle/10635/106313
ISSN: 10196439
DOI: 10.3892/ijo.2013.1843
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

30
checked on Apr 3, 2018

WEB OF SCIENCETM
Citations

26
checked on Apr 3, 2018

Page view(s)

33
checked on Apr 20, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.