Please use this identifier to cite or link to this item:
|Title:||Quantitation of paclitaxel in micro-sample rat plasma by a sensitive reversed-phase HPLC assay|
|Authors:||Wang, L.Z. |
|Source:||Wang, L.Z., Ho, P.C., Lee, H.S., Vaddi, H.K., Chan, Y.W., Yung, C.S. (2003-02-26). Quantitation of paclitaxel in micro-sample rat plasma by a sensitive reversed-phase HPLC assay. Journal of Pharmaceutical and Biomedical Analysis 31 (2) : 283-289. ScholarBank@NUS Repository. https://doi.org/10.1016/S0731-7085(02)00611-8|
|Abstract:||A sensitive high-performance liquid chromatographic (HPLC) method was developed for the determination of paclitaxel in micro-samples of rat plasma in order to study the mechanism of enhanced systemic exposure of paclitaxel co-administered with P-glycoprotein inhibitors. The assay involved solid-phase extraction procedures using 2′-methylpaclitaxel as the internal standard. Chromatographic separations were achieved using a ZORBAX ODS C18 column and mobile phase consisting of acetonitrile, methanol and ammonium acetate buffer (10 mM, pH 5.0) (48.5:16.5:35) pumped at 0.8 ml/min. The effluents were measured for UV absorption at 227 nm, with retention times of 8.5 and 11.0 min for paclitaxel and 2′-methylpaclitaxel, respectively. The chromatographic separation was excellent, with no endogenous interference. The standard curves showed a good linearity (r=0.9994) over the concentration ranges of 10-1000 ng/ml. At 1000 ng/ml, the absolute recoveries of paclitaxel and 2′-methylpaclitaxel are 89 and 90%, respectively. The intra- and inter-day variabilities of paclitaxel were both less than 15%. This validated method for the assay of paclitaxel in micro-sample rat plasma made it feasible to study the pharmacokinetics of the drug in a single rat. © 2002 Elsevier Science B.V. All rights reserved.|
|Source Title:||Journal of Pharmaceutical and Biomedical Analysis|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Mar 7, 2018
WEB OF SCIENCETM
checked on Mar 7, 2018
checked on Feb 25, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.