Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.jpba.2008.09.018
DC Field | Value | |
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dc.title | Quantification of arsenic compounds using derivatization, solvent extraction and liquid chromatography electrospray ionization tandem mass spectrometry | |
dc.contributor.author | Huang, M. | |
dc.contributor.author | Wang, Y.-t. | |
dc.contributor.author | Ho, P.C. | |
dc.date.accessioned | 2014-10-29T01:57:38Z | |
dc.date.available | 2014-10-29T01:57:38Z | |
dc.date.issued | 2008-12-15 | |
dc.identifier.citation | Huang, M., Wang, Y.-t., Ho, P.C. (2008-12-15). Quantification of arsenic compounds using derivatization, solvent extraction and liquid chromatography electrospray ionization tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis 48 (5) : 1381-1391. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jpba.2008.09.018 | |
dc.identifier.issn | 07317085 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/106273 | |
dc.description.abstract | The difficulty in detecting inorganic arsenic compounds using conventional settings of mass chromatography has led to the use of derivatizing agents to aid in their detection. A recent study indicated that 2,3-dimercaptopropanol (BAL) could be used to derivatize arsenic compounds to make them detectable by LC coupled to UV detector. A speciation analysis method was then developed for arsenic compounds after derivatization using the LC-MS/MS with the aim to improve the sensitivity and specificity. The arsenic compounds were derivatized with BAL before solvent extraction was carried out. The resultant extract was analyzed using the LC-MS/MS. However, our finding showed that BAL, being a thiol, reduced the pentavalent arsenic compounds, As(V) to a trivalent state, As(III). The arsenic metabolites, monomethyl arsonate (MMA) and dimethyl arsenic acid (DMA) could also be reduced by BAL to As(V)- and As(III)-BAL adducts. Despite this, the assay could be used to quantify the total arsenic concentration as a summation of all these adducts, when speciation of individual arsenic species is not required. The developed LC-MS/MS assay was subsequently applied to detect arsenic compounds in rat urine samples after oral administration of arsenic trioxide. © 2008 Elsevier B.V. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jpba.2008.09.018 | |
dc.source | Scopus | |
dc.subject | Arsenic | |
dc.subject | BAL | |
dc.subject | Derivatization | |
dc.subject | LC-MS/MS | |
dc.type | Article | |
dc.contributor.department | PHARMACY | |
dc.description.doi | 10.1016/j.jpba.2008.09.018 | |
dc.description.sourcetitle | Journal of Pharmaceutical and Biomedical Analysis | |
dc.description.volume | 48 | |
dc.description.issue | 5 | |
dc.description.page | 1381-1391 | |
dc.description.coden | JPBAD | |
dc.identifier.isiut | 000262920800016 | |
Appears in Collections: | Staff Publications |
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