Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jpba.2008.09.018
Title: Quantification of arsenic compounds using derivatization, solvent extraction and liquid chromatography electrospray ionization tandem mass spectrometry
Authors: Huang, M. 
Wang, Y.-t.
Ho, P.C. 
Keywords: Arsenic
BAL
Derivatization
LC-MS/MS
Issue Date: 15-Dec-2008
Citation: Huang, M., Wang, Y.-t., Ho, P.C. (2008-12-15). Quantification of arsenic compounds using derivatization, solvent extraction and liquid chromatography electrospray ionization tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis 48 (5) : 1381-1391. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jpba.2008.09.018
Abstract: The difficulty in detecting inorganic arsenic compounds using conventional settings of mass chromatography has led to the use of derivatizing agents to aid in their detection. A recent study indicated that 2,3-dimercaptopropanol (BAL) could be used to derivatize arsenic compounds to make them detectable by LC coupled to UV detector. A speciation analysis method was then developed for arsenic compounds after derivatization using the LC-MS/MS with the aim to improve the sensitivity and specificity. The arsenic compounds were derivatized with BAL before solvent extraction was carried out. The resultant extract was analyzed using the LC-MS/MS. However, our finding showed that BAL, being a thiol, reduced the pentavalent arsenic compounds, As(V) to a trivalent state, As(III). The arsenic metabolites, monomethyl arsonate (MMA) and dimethyl arsenic acid (DMA) could also be reduced by BAL to As(V)- and As(III)-BAL adducts. Despite this, the assay could be used to quantify the total arsenic concentration as a summation of all these adducts, when speciation of individual arsenic species is not required. The developed LC-MS/MS assay was subsequently applied to detect arsenic compounds in rat urine samples after oral administration of arsenic trioxide. © 2008 Elsevier B.V. All rights reserved.
Source Title: Journal of Pharmaceutical and Biomedical Analysis
URI: http://scholarbank.nus.edu.sg/handle/10635/106273
ISSN: 07317085
DOI: 10.1016/j.jpba.2008.09.018
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