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|Title:||Quantification of arsenic compounds using derivatization, solvent extraction and liquid chromatography electrospray ionization tandem mass spectrometry|
|Authors:||Huang, M. |
|Citation:||Huang, M., Wang, Y.-t., Ho, P.C. (2008-12-15). Quantification of arsenic compounds using derivatization, solvent extraction and liquid chromatography electrospray ionization tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis 48 (5) : 1381-1391. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jpba.2008.09.018|
|Abstract:||The difficulty in detecting inorganic arsenic compounds using conventional settings of mass chromatography has led to the use of derivatizing agents to aid in their detection. A recent study indicated that 2,3-dimercaptopropanol (BAL) could be used to derivatize arsenic compounds to make them detectable by LC coupled to UV detector. A speciation analysis method was then developed for arsenic compounds after derivatization using the LC-MS/MS with the aim to improve the sensitivity and specificity. The arsenic compounds were derivatized with BAL before solvent extraction was carried out. The resultant extract was analyzed using the LC-MS/MS. However, our finding showed that BAL, being a thiol, reduced the pentavalent arsenic compounds, As(V) to a trivalent state, As(III). The arsenic metabolites, monomethyl arsonate (MMA) and dimethyl arsenic acid (DMA) could also be reduced by BAL to As(V)- and As(III)-BAL adducts. Despite this, the assay could be used to quantify the total arsenic concentration as a summation of all these adducts, when speciation of individual arsenic species is not required. The developed LC-MS/MS assay was subsequently applied to detect arsenic compounds in rat urine samples after oral administration of arsenic trioxide. © 2008 Elsevier B.V. All rights reserved.|
|Source Title:||Journal of Pharmaceutical and Biomedical Analysis|
|Appears in Collections:||Staff Publications|
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