Please use this identifier to cite or link to this item: https://doi.org/10.1002/rcm.2924
Title: Pharmacokinetic study of intraperitoneally administered troglitazone in mice using ultra-performance liquid chromatography/tandem mass spectrometry
Authors: New, L.-S. 
Saha, S.
Ong, M.M.K.
Boelsterli, U.A.
Chan, E.C.Y. 
Issue Date: 2007
Source: New, L.-S., Saha, S., Ong, M.M.K., Boelsterli, U.A., Chan, E.C.Y. (2007). Pharmacokinetic study of intraperitoneally administered troglitazone in mice using ultra-performance liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry 21 (6) : 982-988. ScholarBank@NUS Repository. https://doi.org/10.1002/rcm.2924
Abstract: A rapid and sensitive ultra-performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS) method was developed and validated for the determination of troglitazone in mouse plasma. Troglitazone and its internal standard (IS), rosiglitazone, were separated on an ACQUITY UPLC BEH C 18 column (1.7 μm particle size, 50 X 2.1 mm i.d.) by gradient elution with water and methanol at a flow rate of 0.5 mL/min. The cycle time of each analysis was 2.5 min. Rosiglitazone and troglitazone eluted at 1.13 and 1.57 min, respectively, and were chromatographically resolved from the ion suppression and enhancement zones due to the biological matrix effect. Quantitation of the analytes was performed in electrospray negative ionization mode (ESI -ve) using multiple reaction monitoring (MRM) experiments. The weighted (1/x) calibration curve was quadratic over the plasma concentration range 1-2500 ng/mL with a correlation coefficient (r2) of 0.9966. The limit of quantitation (LOQ) of troglitazone in mouse plasma was lower than 1 ng/mL. The inter- and intra-day variations of the assay were lower than 12.1%; the overall accuracy ranged from 86.4-110.2% and recovery from spiked plasma was more than 60%. The developed method was successfully applied to determine troglitazone in mouse plasma after intraperitoneal administration. Copyright © 2007 John Wiley & Sons, Ltd.
Source Title: Rapid Communications in Mass Spectrometry
URI: http://scholarbank.nus.edu.sg/handle/10635/106207
ISSN: 09514198
DOI: 10.1002/rcm.2924
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