Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/106026
Title: Impact of GSTM1 and GSTP1 gene polymorphisms on erythrocyte GST activity
Authors: Zhong, S.-L.
Wang, Y.-X.
Zhou, S.-F. 
Huang, M.
Keywords: Erythrocyte
Glutathione S-transferase
GST activity
Han Chinese
Polymorphism
Spectrophotometric method
Issue Date: Jun-2005
Source: Zhong, S.-L.,Wang, Y.-X.,Zhou, S.-F.,Huang, M. (2005-06). Impact of GSTM1 and GSTP1 gene polymorphisms on erythrocyte GST activity. Chinese Pharmacological Bulletin 21 (6) : 726-729. ScholarBank@NUS Repository.
Abstract: Aim: The current study was conducted to investigate the effect of GSTP1 codon 105 polymorphism, alone and in combination with GSTM1-deletion polymorphism, on erythrocyte GST activity in 196 Han Chinese. Methods: GST activity was measured in healthy Chinese by a spectrophotometric method (n = 196; 101 males and 95 females; age range 21-81 years; median 43.5 years). GSTM1 polymorphisms were analyzed by a PCR-Multiplex procedure, whereas GSTP1 polymorphism was analyzed by PCR-RFLP. Results: The frequency of GSTM1 null genotype was 56.1% and the frequency of I/I, I/V, and V/V genotypes was 60.7%, 35.2% and 4.1%, respectively, in Han Chinese. The mean erythrocyte GST enzyme activity for I/V genotype group(3.53 ± 0.63 U · g-1 Hb) was significantly lower than that for I/I genotypes (4.25 ± 1.07 U · g-1 Hb, P = 0.000), while significantly higher than that for V/V genotypes (2.44 ± O. 67 U · g-1 Hb, P = 0.004). In GSTM1 (-) group, the GST activity of carriers of GSTM1 (-)/ GSTP1 - I/I is significantly higher than that of GSTM1 (-)/GSTP1 - I/V or-V/V, however, in GSTM1 (+) group, there is no difference between different subgroups. There was no significant difference in the mean GST activity among different age groups. Erythrocyte GST activities were significantly higher in females than in males, but not significantly. Conclusion: The GST activity measured by CDNB-based assay is probably strongly correlated with the GSTP1 105Val genotype, although other GST enzymes would tend to dilute the GSTP1 genotype effect.
Source Title: Chinese Pharmacological Bulletin
URI: http://scholarbank.nus.edu.sg/handle/10635/106026
ISSN: 10011978
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

28
checked on Feb 17, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.