Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jchromb.2013.08.025
Title: Global gas chromatography/time-of-flight mass spectrometry (GC/TOFMS)-based metabonomic profiling of lyophilized human feces
Authors: Phua, L.C.
Koh, P.K.
Cheah, P.Y.
Ho, H.K. 
Chan, E.C.Y. 
Keywords: Colorectal cancer
Feces
Gas chromatography mass spectrometry
Metabolic profiling
Metabolomics
Metabonomics
Issue Date: 15-Oct-2013
Citation: Phua, L.C., Koh, P.K., Cheah, P.Y., Ho, H.K., Chan, E.C.Y. (2013-10-15). Global gas chromatography/time-of-flight mass spectrometry (GC/TOFMS)-based metabonomic profiling of lyophilized human feces. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 937 : 103-113. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jchromb.2013.08.025
Abstract: Gas chromatography mass spectrometry (GC/MS)-based fecal metabonomics represents a powerful systems biology approach for elucidating metabolic biomarkers of lower gastrointestinal tract (GIT) diseases. Unlike metabolic profiling of fecal water, the profiling of complete fecal material remains under-explored. Here, a gas chromatography/time-of-flight mass spectrometry (GC/TOFMS) method was developed and validated for the global metabonomic profiling of human feces. Fecal and fecal water metabotypes were also profiled and compared. Additionally, the unclear influence of blood in stool on the fecal metabotype was investigated unprecedentedly. Eighty milligram of lyophilized feces was ultrasonicated with 1mL of methanol:water (8:2) for 30min, followed by centrifugation, drying of supernatant, oximation and trimethylsilylation for 45min. Lyophilized feces demonstrated a more comprehensive metabolic coverage than fecal water, based on the number of chromatographic peaks. Principal component analysis (PCA) indicated occult blood (1mgHb/g feces) exerted a negligible effect on the fecal metabotype. Conversely, a unique metabotype related to feces spiked with gross blood (100mgHb/g feces) was revealed (PCA, R2X=0.837, Q2=0.794), confirming the potential confounding effect of gross GIT bleeding on the fecal metabotype. This pertinent finding highlights the importance of prudent interpretation of fecal metabonomic data, particularly in GIT diseases where bleeding is prevalent. © 2013 Elsevier B.V.
Source Title: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
URI: http://scholarbank.nus.edu.sg/handle/10635/105982
ISSN: 15700232
DOI: 10.1016/j.jchromb.2013.08.025
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