Please use this identifier to cite or link to this item: https://doi.org/10.1021/nn300352b
Title: Frequency shifts in SERS for biosensing
Authors: Kho, K.W.
Dinish, U.S.
Kumar, A.
Olivo, M. 
Keywords: frequency shift
label-free biodetection
protein
sensor
SERS
stress
Issue Date: 26-Jun-2012
Citation: Kho, K.W., Dinish, U.S., Kumar, A., Olivo, M. (2012-06-26). Frequency shifts in SERS for biosensing. ACS Nano 6 (6) : 4892-4902. ScholarBank@NUS Repository. https://doi.org/10.1021/nn300352b
Abstract: Figure Persented: We report an observation of a peculiar effect in which the vibrational frequencies of antibody-conjugated SERS-active reporter molecules are shifted in quantitative correlation with the concentration of the targeted antigen. We attribute the frequency shifts to mechanical perturbations in the antibody-reporter complex, as a result of antibody-antigen interaction forces. Our observation thus demonstrates the potentiality of an antibody-conjugated SERS-active reporter complex as a SERS-active nanomechanical sensor for biodetection. Remarkably, our sensing scheme, despite employing only one antibody, was found to be able to achieve detection sensitivity comparable to that of a conventional sandwich immunoassay. Additionally, we have carried out a proof-of-concept study into using multiple "stress-sensitive" SERS reporters for multiplexed detection of antigen-antibody bindings at the subdiffraction limit. The current work could therefore pave the way to realizing a label-free high-density protein nanoarray. © 2012 American Chemical Society.
Source Title: ACS Nano
URI: http://scholarbank.nus.edu.sg/handle/10635/105968
ISSN: 19360851
DOI: 10.1021/nn300352b
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