Please use this identifier to cite or link to this item:
|Title:||Development and characterization of an immobilized human organic cation transporter based liquid chromatographic stationary phase|
|Citation:||Moaddel, R., Yamaguchi, R., Ho, P.C., Patel, S., Hsu, C.-P., Subrahmanyam, V., Wainer, I.W. (2005-04-25). Development and characterization of an immobilized human organic cation transporter based liquid chromatographic stationary phase. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 818 (2) : 263-268. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jchromb.2005.01.015|
|Abstract:||Membranes from a stably transfected cell line that expresses the human organic cation 1 transporter (hOCT1) have been immobilized on the immobilized artificial membrane (IAM) liquid chromatographic stationary phase to form the hOCT1(+)-IAM stationary phase. Membranes from the parent cell line that does not express the hOCT1 were also immobilized to create the hOCT1(-)-IAM stationary phase. Columns were created using both stationary phases, and frontal displacement chromatography experiments were conducted using [ 3H]-methyl phenyl pyridinium ([3H]-MPP+) as the marker ligand and MPP+, verapamil, quinidine, quinine, nicotine, dopamine and vinblastin as the displacers. The Kd values calculated from the chromatographic studies correlated with previously reported K i values (r2 = 0.9987; p < 0.001). The data indicate that the hOCT1(+)-IAM column can be used for the on-line determination of binding affinities to the hOCT1 and that these affinities are comparable to those obtained using cellular uptake studies. In addition, the chromatographic method was able to identify a previously undetected high affinity binding site for MPP+ and to determine that hOCT1 bound (R)-verapamil to a greater extent than (S)-verapamil.|
|Source Title:||Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jan 12, 2019
WEB OF SCIENCETM
checked on Jan 2, 2019
checked on Dec 7, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.