Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.jpba.2005.02.041
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dc.title | Determination of thalidomide by high performance liquid chromatography: Plasma pharmacokinetic studies in the rat | |
dc.contributor.author | Yang, X. | |
dc.contributor.author | Hu, Z. | |
dc.contributor.author | Sui, Y.C. | |
dc.contributor.author | Ho, P.C. | |
dc.contributor.author | Chan, E. | |
dc.contributor.author | Duan, W. | |
dc.contributor.author | Boon, C.G. | |
dc.contributor.author | Zhou, S. | |
dc.date.accessioned | 2014-10-29T01:51:01Z | |
dc.date.available | 2014-10-29T01:51:01Z | |
dc.date.issued | 2005-09-01 | |
dc.identifier.citation | Yang, X., Hu, Z., Sui, Y.C., Ho, P.C., Chan, E., Duan, W., Boon, C.G., Zhou, S. (2005-09-01). Determination of thalidomide by high performance liquid chromatography: Plasma pharmacokinetic studies in the rat. Journal of Pharmaceutical and Biomedical Analysis 39 (1-2) : 299-304. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jpba.2005.02.041 | |
dc.identifier.issn | 07317085 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/105806 | |
dc.description.abstract | A sensitive and simple high performance liquid chromatography (HPLC) method was developed and validated for the determination of thalidomide in rat plasma. Chromatography was accomplished with a reversed-phase Hypersil C18 column. Mobile phase consisted of acetonitrile-10 mM ammonium acetate buffer (pH 5.50) (28:72, v/v), at a flow rate of 0.8 ml/min. Thalidomide was monitored by ultraviolet detector at 220 nm and it gave a linear response as a function of concentration over 0.02-50 μM. The limit of quantitation in rat plasma was 0.50 ng (0.02 μM plasma concentration) with an aliquot of 20 μl. Results from a 3-day validation study indicated that this method allows for simple and rapid quantitation of thalidomide with excellent accuracy and reliability. Using this validated assay, the effect of coadministered irinotecan (CPT-11) on the plasma pharmacokinetics of thalidomide in rats was determined. Coadministration of CPT-11 (intravenously, 60 mg/kg) increased the maximum plasma concentration (Cmax) and area under the plasma concentration-time curve (AUC 0-10 h) of thalidomide by 32.29 and 11.66%, respectively, as compared to the control, but none of the effect of CPT-11 was of statistical significance (P > 0.05). Concomitant CPT-11 also caused a 10.04% decrease in plasma clearance (CL) and 14.51% decrease in volume of distribution (V d) (P > 0.05). These results suggest that coadministered CPT-11 did not significantly alter the plasma pharmacokinetics of thalidomide in rats. Further studies are warranted to explore the pharmacokinetic and pharmacodynamic interactions between CPT-11 and thalidomide. © 2005 Elsevier B.V. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jpba.2005.02.041 | |
dc.source | Scopus | |
dc.subject | HPLC | |
dc.subject | Pharmacokinetics | |
dc.subject | Thalidomide | |
dc.type | Article | |
dc.contributor.department | PHARMACY | |
dc.description.doi | 10.1016/j.jpba.2005.02.041 | |
dc.description.sourcetitle | Journal of Pharmaceutical and Biomedical Analysis | |
dc.description.volume | 39 | |
dc.description.issue | 1-2 | |
dc.description.page | 299-304 | |
dc.description.coden | JPBAD | |
dc.identifier.isiut | 000232209600042 | |
Appears in Collections: | Staff Publications |
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