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Title: Cell confinement in patterned nanoliter droplets in a microwell array by wiping
Authors: Kang, L. 
Hancock, M.J.
Brigham, M.D.
Khademhosseini, A.
Keywords: Cell seeding
Microwell arrays
Nanoliter droplets
Issue Date: May-2010
Citation: Kang, L., Hancock, M.J., Brigham, M.D., Khademhosseini, A. (2010-05). Cell confinement in patterned nanoliter droplets in a microwell array by wiping. Journal of Biomedical Materials Research - Part A 93 (2) : 547-557. ScholarBank@NUS Repository.
Abstract: Cell patterning is useful for a variety of biological applications such as tissue engineering and drug discovery. In particular, the ability to localize cells within distinct fluids is beneficial for a variety of applications ranging from microencapsulation to high-throughput analysis. However, despite much progress, cell immobilization and maintenance within patterned microscale droplets remains a challenge. In particular, no method currently exists to rapidly seed cells into microwell arrays in a controllable and reliable manner. In this study, we present a simple wiping technique to localize cells within arrays of polymeric microwells. This robust method produces cell seeding densities that vary consistently with microwell geometry and cell concentration. Moreover, we develop a simple theoretical model to accurately predict cell seeding density and seeding ef.-ciency in terms of the design parameters of the microwell array and the cell density. This short-term cell patterning approach is an enabling tool to develop new high-throughput screening technologies that utilize microwell arrays containing cells for screening applications. © 2009 Wiley Periodicals, Inc.
Source Title: Journal of Biomedical Materials Research - Part A
ISSN: 15493296
DOI: 10.1002/jbm.a.32557
Appears in Collections:Staff Publications

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